LOCUS       sequence-223803-        8469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of yeVenus and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW yeVenus-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             835..1548
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1555..1608
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1624..1633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1630..1632,1633..1635,1636..2349)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1675..1696)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1936..1955)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2283..2304
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2359..2383)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2445..2464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2446..2464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2612..2799
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2667..2691)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2811..2828)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2811..2827)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2820..2842)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3263..4609
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4720..5523)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5524..5739)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5764..5782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5850..5954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5955..6023,6024..6815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6173..6192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6986..7574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7475..7494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7728..7745
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7862..7883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7898..7903,7904..7921,7922..7928)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7936..7952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7941..7963
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7960..7976
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7960..7976
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8006..8461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8143..8164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8355..8374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc caccatgtct
      841 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
      901 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
      961 accttaaaat tgatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     1021 actttaggtt atggtttgca atgttttgct agatacccag atcatatgaa acaacatgac
     1081 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     1141 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     1201 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     1261 tacaactata actctcacaa tgtttacatc actgctgaca aacaaaagaa tggtatcaaa
     1321 gctaacttca aaattagaca caacattgaa gatggtggtg ttcaattagc tgaccattat
     1381 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     1441 tatcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     1501 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaatc cggagagggc
     1561 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg ggatccaccg
     1621 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1681 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1741 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1801 tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac
     1861 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1921 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1981 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2041 ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac cgccgacaag
     2101 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     2161 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2221 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     2281 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2341 tacaagtaaa gcggccgcac ccagctttct tgtacaaagt ggtgtagtta attcatgatg
     2401 gccgctgcag gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
     2461 attacgcgcg gatccagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     2521 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     2581 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     2641 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     2701 aaaacganaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     2761 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccaattc actggccgtc
     2821 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2881 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2941 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     3001 tgcggtattt cacaccgcat aatcggatcg tacttgttac ccatcattga attttgaaca
     3061 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
     3121 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
     3181 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
     3241 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     3301 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     3361 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     3421 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     3481 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     3541 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     3601 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     3661 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     3721 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     3781 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     3841 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     3901 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     3961 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     4021 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     4081 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     4141 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     4201 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
     4261 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     4321 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     4381 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     4441 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     4501 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
     4561 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
     4621 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
     4681 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     4741 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     4801 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     4861 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     4921 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     4981 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     5041 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     5101 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     5161 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     5221 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     5281 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     5341 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     5401 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     5461 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     5521 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
     5581 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     5641 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     5701 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agctaattct tgaagacgaa
     5761 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5821 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6001 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6061 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6121 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6181 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6241 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6301 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6361 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6421 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6481 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6661 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7681 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7741 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7801 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7861 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7921 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7981 attacgccaa gcttaccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     8041 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     8101 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     8161 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     8221 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     8281 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     8341 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     8401 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     8461 tccattcgc
//
