LOCUS       sequence-223823-        4919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mVenus with CaUra3 selection. *An updated version of this plasmid is
            available: #168058 pFA6a-link-ymVenus-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ymVenus-CaURA3
FEATURES             Location/Qualifiers
     source          1..4919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1105..1821
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1862..2049
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1917..1941)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2124..2467
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3286..3483
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3590..3609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3591..3609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3696..3713)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3867..4455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3947..3966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4626..4919,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaaatgtct aaaggtgaag aattattcac tggtgttgtc
     1141 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     1201 ggtgaaggtg atgctactta cggtaaattg accttaaaat tgatttgtac tactggtaaa
     1261 ttgccagttc catggccaac cttagtcact actttaggtt atggtttgca atgttttgct
     1321 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     1381 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     1441 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     1501 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     1561 actgctgaca aacaaaagaa tggtatcaaa gctaacttca aaattagaca caacattgaa
     1621 gatggtggtg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     1681 gtcttgttac cagacaacca ttacttatcc tatcaatcta aattatccaa agatccaaac
     1741 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     1801 atggatgaat tgtacaaata agcggccgcc tcgaggtcga cggcgcgcca cttctaaata
     1861 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     1921 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     1981 tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac
     2041 ctctaccggc agatccgcta gggataacag ggtaatatag atctgtttag cttgcctcgt
     2101 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
     2161 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
     2221 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
     2281 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
     2341 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
     2401 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
     2461 aacaaccatg acagtcaaca ctaagaccta tagtgagaga gcagaaactc atgcctcacc
     2521 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
     2581 tgacgttgat accactaagg aattccttga attaattgat aaattaggtc cttatgtatg
     2641 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
     2701 attattagaa ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga
     2761 tattggtaat accgtaaaga aacaatatat tggtggagtt tataaaatta gtagttgggc
     2821 agatattacc aatgctcatg gtgtcactgg gaatggagtg gttgaaggat taaaacaggg
     2881 agctaaagaa accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc
     2941 atcagtggga tcattagcat atggagaata ttctcaaaaa actgttgaaa ttgctaaatc
     3001 cgataaggaa tttgttattg gatttattgc ccaacgtgat atgggtggcc aagaagaagg
     3061 atttgattgg cttattatga cacctggagt tggattagat gataaaggtg atggattagg
     3121 acaacaatat agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg
     3181 tagaggattg tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagaaatgc
     3241 tggttggaat gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag
     3301 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     3361 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     3421 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     3481 ctgctgtcga ttcgatacta acgccgccat ccagtgtcga aaacgagctc gaattcatcg
     3541 atgatatcag atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga
     3601 gtcgtattaa tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg
     3661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     4081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagttt
//
