LOCUS       sequence-223824-        4922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with ymYPET with CaUra3
            selection. *An updated version of this plasmid is available: #168056
            pFA6a-link-ymYPET-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ymYPET-CaURA3
FEATURES             Location/Qualifiers
     source          1..4922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1865..2052
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1920..1944)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2127..2470
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3289..3486
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3593..3612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3594..3612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3699..3716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3870..4458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3950..3969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4629..4922,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaaatggtc tccaagggcg aagaattgtt caccggtgtg
     1141 gtcccaatct tggtcgagtt ggacggtgac gtcaacggtc acaagttctc tgtttccggt
     1201 gaaggtgaag gagacgccac ctacggtaaa ttgaccttga agttgttgtg caccaccggc
     1261 aagctgccag ttccatggcc aacattggtc accactttag gttatggttt acaatgtttt
     1321 gctagatatc cagatcatat gaaacaacat gatttcttca aatctgctat gccagaaggt
     1381 tatgttcaag aaagaactat atttttcaaa gatgatggta attataaaac tagagctgaa
     1441 gttaaatttg aaggagatac tttagttaat agaattgaat tgaaaggtat tgattttaaa
     1501 gaagatggta atattttagg tcataaatta gaatataatt ataattctca taatgtttat
     1561 attacagctg ataaacaaaa gaatggtatt aaggctaatt ttaaaattag acataatatt
     1621 gaagatggtg gtgttcaatt agctgatcat tatcaacaaa atactcctat tggtgacggt
     1681 ccagttttat tacctgataa tcattatttg tcatatcaat ctaaattgtc aaaagatcca
     1741 aatgaaaaaa gagatcatat ggttttgtta gaatttttga ctgcagctgg tattacttta
     1801 ggtatggatg aattgtataa ataagcggcc gcctcgaggt cgacggcgcg ccacttctaa
     1861 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     1921 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     1981 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
     2041 cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct
     2101 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
     2161 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
     2221 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
     2281 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
     2341 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
     2401 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
     2461 ataaacaacc atgacagtca acactaagac ctatagtgag agagcagaaa ctcatgcctc
     2521 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
     2581 aattgacgtt gataccacta aggaattcct tgaattaatt gataaattag gtccttatgt
     2641 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
     2701 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     2761 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     2821 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     2881 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     2941 atcatcagtg ggatcattag catatggaga atattctcaa aaaactgttg aaattgctaa
     3001 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     3061 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     3121 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     3181 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     3241 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     3301 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     3361 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     3421 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     3481 atactgctgt cgattcgata ctaacgccgc catccagtgt cgaaaacgag ctcgaattca
     3541 tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag
     3601 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg
     3661 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3841 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3901 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3961 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4021 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     4081 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4141 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4201 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4261 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     4321 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4381 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4441 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4501 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4561 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4621 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4681 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4741 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4801 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4861 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4921 tt
//
