LOCUS       sequence-223825-        5633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            tdTomato with CaUra3 selection. *An updated version of this plasmid 
            is available: #168054 pFA6a-link-ytdTomato-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ytdTomato-CaURA3
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      2576..2763
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2631..2655)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2838..3181
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      4000..4197
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4304..4323)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4305..4323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4410..4427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4581..5169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4661..4680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5340..5633,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaaatggtt agtaaaggtg aggaagtgat taaggaattc
     1141 atgaggttta aagttagaat ggaaggttct atgaatggtc atgaatttga aattgaaggt
     1201 gaaggtgaag gtagaccata tgaaggtaca caaactgcta aattgaaagt tacaaaaggt
     1261 ggtccattac catttgcatg ggatatttta tctccacagt ttatgtatgg tagtaaagca
     1321 tatgttaaac atccagctga tattccagat tataagaaat taagttttcc tgaaggtttt
     1381 aaatgggaaa gagtaatgaa ttttgaagat ggtggtttag taacagttac tcaagattct
     1441 tctttacaag atggtacatt aatatataaa gttaaaatga gaggtactaa ttttccacct
     1501 gatggtccag ttatgcagaa gaaaacaatg ggttgggaag cctcaactga aagattgtac
     1561 cctagagatg gagttttgaa aggtgaaatt catcaagctc ttaagttaaa agatggaggt
     1621 cactatttgg ttgagtttaa aactatatac atggctaaaa aacctgttca attgccaggt
     1681 tattattatg ttgatactaa attagatatt acttcacata atgaagatta tactattgtt
     1741 gaacaatatg aaagatctga aggtagacat catttgtttt tgggtcatgg tactggtagt
     1801 actggttctg gttcttcagg tactgcttct agtgaagata ataatatggc tgtaatcaaa
     1861 gagttcatga ggtttaaagt tagaatggaa ggttctatga atggtcatga gtttgaaatc
     1921 gaaggtgaag gagaaggtag accatatgaa ggtactcaaa ctgctaaatt gaaagttact
     1981 aaaggtggtc cattgccatt tgcttgggat atattgtcac ctcagtttat gtatggttct
     2041 aaagcatatg ttaaacatcc agctgatatt cctgattata aaaaattatc atttcctgaa
     2101 ggttttaaat gggaaagagt tatgaatttt gaagatggtg gtttagttac tgtaactcaa
     2161 gattcttcat tgcaagatgg tacattaata tataaagtta aaatgagagg tactaatttt
     2221 ccacctgatg gtccagttat gcagaaaaag actatgggtt gggaagcatc tactgaaaga
     2281 ttatatccta gagatggtgt tttaaaaggt gaaattcacc aagccttaaa attgaaagat
     2341 ggtggtcatt atttggttga attcaaaaca atctacatgg ctaaaaaacc agttcagctg
     2401 cccggatact attatgtcga tactaaactg gatattactt cacacaatga agactacaca
     2461 attgttgaac agtatgaaag atcagagggt agacatcact tgtttttata cggtatggat
     2521 gaattatata aataagcggc cgcctcgagg tcgacggcgc gccacttcta aataagcgaa
     2581 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     2641 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     2701 gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac
     2761 cggcagatcc gctagggata acagggtaat atagatctgt ttagcttgcc tcgtccccgc
     2821 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     2881 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     2941 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     3001 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     3061 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     3121 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     3181 catgacagtc aacactaaga cctatagtga gagagcagaa actcatgcct caccagtagc
     3241 acagcgatta tttcgattaa tggaactgaa gaaaaccaat ttatgtgcat caattgacgt
     3301 tgataccact aaggaattcc ttgaattaat tgataaatta ggtccttatg tatgcttaat
     3361 caagactcat attgatataa tcaatgattt ttcctatgaa tccactattg aaccattatt
     3421 agaactttca cgtaaacatc aatttatgat ttttgaagat agaaaatttg ctgatattgg
     3481 taataccgta aagaaacaat atattggtgg agtttataaa attagtagtt gggcagatat
     3541 taccaatgct catggtgtca ctgggaatgg agtggttgaa ggattaaaac agggagctaa
     3601 agaaaccacc accaaccaag agccaagagg gttattgatg ttagctgaat tatcatcagt
     3661 gggatcatta gcatatggag aatattctca aaaaactgtt gaaattgcta aatccgataa
     3721 ggaatttgtt attggattta ttgcccaacg tgatatgggt ggccaagaag aaggatttga
     3781 ttggcttatt atgacacctg gagttggatt agatgataaa ggtgatggat taggacaaca
     3841 atatagaact gttgatgaag ttgttagcac tggaactgat attatcattg ttggtagagg
     3901 attgtttggt aaaggaagag atccagatat tgaaggtaaa aggtatagaa atgctggttg
     3961 gaatgcttat ttgaaaaaga ctggccaatt ataatcagta ctgacaataa aaagattctt
     4021 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     4081 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     4141 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     4201 tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctcgaattc atcgatgata
     4261 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     4321 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4741 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag ttt
//
