LOCUS       sequence-223832-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ
  TITLE     Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
  JOURNAL   Nature. 1987 Feb 5-11;325(6104):549-52.
  PUBMED    3027579
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1987 Feb 5-11"; volume: "325(6104)"; pages: "549-52"
COMMENT     Sequence Label: MSCV-human CSF-1R-Y969F,S301-IRES—GFP
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             110..626
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    690..1031
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1098..1514
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1444..1466
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1482..1498
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(4810..5384,5385..5387,5388..5396)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4977..4994)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5204..5223
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5397..5399,5400..5402,5403..6116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5397..5399,5400..5402,5403..6113)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5442..5463)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5703..5722)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6050..6071
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6195..6709
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6871..6887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6895..6901,6902..6919,6920..6925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6940..6961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7078..7095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7249..7837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7329..7348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8008..8799,8800..8868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8631..8650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8869..8973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9041..9059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9097..9119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9219..9238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9432..9454
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9582..9601
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
       61 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
      121 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac
      181 ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg
      241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
      301 ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
      361 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
      421 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
      481 cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt
      541 gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg
      601 actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc
      661 agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct
      721 ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttag
      781 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
      841 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
      901 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
      961 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
     1021 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     1081 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     1141 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     1201 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     1261 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     1321 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     1381 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     1441 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     1501 cttctctagg cgccggaatt cgttaacctc gagagatccg cggccgctga tccgctagag
     1561 tcgacctgca gcccaagcta attcgcatgg tcgactacca gaggagagga acgtgtgtcc
     1621 agtgtcccga tccctgcgga gctagtagct gagagctctg tgccctgggc accttgcagc
     1681 cctgcacctg cctgccactt ccccaccgag gccatgggcc caggagttct gctgctcctg
     1741 ctggtggcca cagcttggca tggtcaggga atcccagtga tagagcccag tgtccccgag
     1801 ctggtcgtga agccaggagc aacggtgacc ttgcgatgtg tgggcaatgg cagcgtggaa
     1861 tgggatggcc ccccatcacc tcactggacc ctgtactctg atggctccag cagcatcctc
     1921 agcaccaaca acgctacctt ccaaaacacg gggacctatc gctgcactga gcctggagac
     1981 cccctgggag gcagcgccgc catccacctc tatgtcaaag accctgcccg gccctggaac
     2041 gtgctagcac aggaggtggt cgtgttcgag gaccaggacg cactactgcc ctgtctgctc
     2101 acagacccgg tgctggaagc aggcgtctcg ctggtgcgtg tgcgtggccg gcccctcatg
     2161 cgccacacca actactcctt ctcgccctgg catggcttca ccatccacag ggccaagttc
     2221 attcagagcc aggactatca atgcagtgcc ctgatgggtg gcaggaaggt gatgtccatc
     2281 agcatccggc tgaaagtgca gaaagtcatc ccagggcccc cagccttgac actggtgcct
     2341 gcagagctgg tgcggattcg aggggaggct gcccagatcg tgtgctcagc cagcagcgtt
     2401 gatgttaact ttgatgtctt cctccaacac aacaacacta agctcgcaat ccctcaacaa
     2461 tctgactttc ataataaccg ttaccaaaaa gtcctgaccc tcaacctcga tcaagtagat
     2521 ttccaacatg ccggcaacta ctcctgcgtg gccagcaacg tgcagggcaa gcactccacc
     2581 tccatgttct tccgggtggt agagagtgcc tactcgaact tgagctctga gcagaacctc
     2641 atccaggagg tgaccgtggg ggaggggctc aacctcaaag tcatggtgga ggcctaccca
     2701 ggcctgcaag gttttaactg gacctacctg ggaccctttt ctgaccacca gcctgagccc
     2761 aagcttgcta atgctaccac caaggacaca tacaggcaca ccttcaccct ctctctgccc
     2821 cgcctgaagc cctctgaggc tggccgctac tccttcctgg ccagaaaccc aggaggctgg
     2881 agagctctga cgtttgagct cacccttcga taccccccag aggtaagcgt catatggaca
     2941 ttcatcaacg gctctggcac ccttttgtgt gctgcctctg ggtaccccca gcccaacgtg
     3001 acatggctgc agtgcagtgg ccacactgat aggtgtgatg aggcccaagt gctgcaggtc
     3061 tgggatgacc cataccctga ggtcctgagc caggagccct tccacaaggt gacggtgcag
     3121 agcctgctga ctgttgagac cttagagcac aaccaaacct acgagtgcag ggcccacaac
     3181 agcgtgggga gtggctcctg ggccttcata cccatctctg caggagccca cacgcatccc
     3241 ccggatgagt tcctcttcac accagtggtg gtcgcctgca tgtccatcat ggccttgctg
     3301 ctgctgctgc tcctgctgct attgtacaag tataagcaga agcccaagta ccaggtccgc
     3361 tggaagatca tcgagagcta tgagggcaac agttatactt tcatcgaccc cacgcagctg
     3421 ccttacaacg agaagtggga gttcccccgg aacaacctgc agtttggtaa gaccctcgga
     3481 gctggagcct ttgggaaggt ggtggaggcc acggcctttg gtctgggcaa ggaggatgct
     3541 gtcctgaagg tggctgtgaa gatgctgaag tccacggccc atgctgatga gaaggaggcc
     3601 ctcatgtccg agctgaagat catgagccac ctgggccagc acgagaacat cgtcaacctt
     3661 ctgggagcct gtacccatgg aggccctgta ctggtcatca cggagtactg ttgctatggc
     3721 gacctgctca actttctgcg aaggaaggct gaggccatgc tgggacccag cctgagcccc
     3781 ggccaggacc ccgagggagg cgtcgactat aagaacatcc acctcgagaa gaaatatgtc
     3841 cgcagggaca gtggcttctc cagccagggt gtggacacct atgtggagat gaggcctgtc
     3901 tccacttctt caaatgactc cttctctgag caagacctgg acaaggagga tggacggccc
     3961 ctggagctcc gggacctgct tcacttctcc agccaagtag cccagggcat ggccttcctc
     4021 gcttccaaga attgcatcca ccgggacgtg gcagcgcgta acgtgctgtt gaccaatggt
     4081 catgtggcca agattgggga cttcgggctg gctagggaca tcatgaatga ctccaactac
     4141 attgtcaagg gcaatgcccg cctgcctgtg aagtggatgg ccccagagag catctttgac
     4201 tgtgtctaca cggttcagag cgacgtctgg tcctatggca tcctcctctg ggagatcttc
     4261 tcacttgggc tgaatcccta ccctggcatc ctggtgaaca gcaagttcta taaactggtg
     4321 aaggatggat accaaatggc ccagcctgca tttgccccaa agaatatata cagcatcatg
     4381 caggcctgct gggccttgga gcccacccac agacccacct tccagcagat ctgctccttc
     4441 cttcaggagc aggcccaaga ggacaggaga gagcgggact ataccaatct gccgagcagc
     4501 agcagaagcg gtggcagcgg cagcagcagc agtgagctgg aggaggagag ctctagtgag
     4561 cacctgacct gctgcgagca aggggatatc gcccagccct tgctgcagcc caacaacttc
     4621 cagttctgct gaggagttga cgacagggag taccactctc ccctcctcca aacttcaact
     4681 cctccatgga tggggcgaca cggggagaac atacaaactc tgccttcggt catttcactc
     4741 aacagctcgg cccagctctg aaacttggga aggtgaggga ttcaggggag gtcagaggat
     4801 cagcggccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4861 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4921 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4981 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5041 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5101 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5161 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     5221 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     5281 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     5341 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     5401 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5461 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5521 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5581 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     5641 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5701 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5761 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5821 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5881 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5941 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6001 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6061 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaactcg
     6121 agggatccgt cgacctgcag ccaagcttat cgataaaata aaagatttta tttagtctcc
     6181 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     6241 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     6301 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     6361 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     6421 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     6481 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     6541 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     6601 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     6661 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt
     6721 tcttgaagtt ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc
     6781 aattagccac tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca
     6841 gcgcagaaag agctggggag aattgtgaaa ttgttatccg ctcacaattc cacacaacat
     6901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6961 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7021 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7681 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9001 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9061 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     9121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     9241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     9301 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     9361 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     9421 gccagggttt tcccagtcac gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag
     9481 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     9541 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     9601 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     9661 cgattagtcc aatttgttaa agacaggata t
//
