LOCUS       sequence-223865-        2605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 5' tagging with the 5' myristoylation 
            sequence from ARF5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: pME-myristo
FEATURES             Location/Qualifiers
     source          1..2605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(162..261)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(278..297)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(279..297)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(302..318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(302..318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(420..439)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             431..1240
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(498..517)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1387..1975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1876..1895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2129..2146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2305..2332)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2424..2510)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2559..2581
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2573..2590
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2574..2590
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct gccgccacca tgggcctgac
      121 catcagcagc ctgttcggcg gaagcggtgg cagcggaggg tacccagctt tcttgtacaa
      181 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      241 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
      301 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      361 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      421 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
      481 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      541 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      601 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
      661 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
      721 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
      781 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
      841 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
      901 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
      961 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     1021 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     1081 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     1141 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     1201 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     1261 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     1321 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1381 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1441 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1501 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1561 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1621 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1681 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1741 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1801 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1861 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1921 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1981 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2041 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2101 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2161 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2221 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
     2281 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
     2341 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     2401 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     2461 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
     2521 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
     2581 gacggccagt cttaagctcg ggccc
//
