LOCUS       sequence-223872-        2800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 5' tagging with the N-terminal sequence
            from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi 
            complex..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: pME-GalT
FEATURES             Location/Qualifiers
     source          1..2800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(357..456)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(473..492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(474..492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(497..513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(497..513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(615..634)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             626..1435
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(693..712)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1582..2170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2071..2090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2324..2341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2500..2527)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2619..2705)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2754..2776
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2768..2785
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2769..2785
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct gccaccatgc cggactccac
      121 cgggaacttc agcctcctgc agcggacctg ctctctggtg gtgctgctgt gcttcttaca
      181 catctttgtc accgtcattt actacatgag gaactcggac tctcggccag ctttcgccca
      241 gaaccagcag cagagaccga cgatacacag gaaactggcg gagcagaggg gcaccactga
      301 ggacagcaga cccgcggcca acgcctcgag caacggccag gagctgcaga tctgcgaccc
      361 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      421 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
      481 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
      541 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
      601 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
      661 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      721 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
      781 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      841 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      901 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
      961 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1021 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     1081 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1141 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     1201 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1261 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1321 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1381 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1441 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     1501 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     1561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2401 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2461 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     2521 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     2581 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     2641 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     2701 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     2761 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
