LOCUS       sequence-223882-        3258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab10
FEATURES             Location/Qualifiers
     source          1..3258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(744..839)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(876..895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(877..895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(900..916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(900..916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1018..1037)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1029..1838
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1096..1115)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1985..2573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2474..2493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2727..2744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2903..2930)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3022..3108)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3157..3179
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3171..3188
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3172..3188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcga agaagaccta tgatctgctc tttaaactgc tcttgattgg cgattctggg
      181 gtgggaaaga cctgcgtgct gttccgattc tccgatgacg ccttcaacac cacctttatc
      241 tccactatag gaatcgattt taagatcaaa acagttgaat tgcaaggaaa gaagataaag
      301 ctacagatat gggatacagc agggcaggaa cggtttcaca ccatcactac ctcttactac
      361 agaggggcaa tgggaatcat gctcgtatat gacatcacca atgcaaagag cttcgaaaac
      421 atcagcaaat ggctcagaaa cattgatgag cacgctaatg aggatgtgga gaggatgctg
      481 ctaggcaaca agtgtgacat ggaggacaaa cgtgtcgtac caaaagccaa gggggaacag
      541 atcgcaaaag aacacgggat tcgcttcttc gagacgagtg caaaagccaa catcaacatc
      601 gagaaggcgt tcctcacgtt agcagaagac attctcaaaa agacacccgt aaaagagcct
      661 aacagtgaaa acgtggacat cagcactgga ggaggagtca caggctggaa gaccaagtgc
      721 tgcagttgaa cacacactca cagcaacttt attatacaaa gttggcatta taaaaaagca
      781 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      841 gagctccatg gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg
      901 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
      961 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1021 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1081 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1141 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1201 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1261 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1321 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     1381 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     1441 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     1501 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     1561 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     1621 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     1681 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     1741 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     1801 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     1861 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     1921 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2761 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2821 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     2881 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     2941 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     3001 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3061 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     3121 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     3181 cggccagtct taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata
     3241 ccatctaagt agttggtt
//
