LOCUS       sequence-223884-        3300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab11al.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab11al
FEATURES             Location/Qualifiers
     source          1..3300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(786..881)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(918..937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(919..937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(942..958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(942..958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1060..1079)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1071..1880
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1138..1157)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2027..2615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2516..2535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2769..2786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2945..2972)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3064..3150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3199..3221
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3213..3230
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3214..3230
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgactg gtagagagga cgagtatgac tatctcttta aagtggttct gattggtgac
      181 tcgggtgtgg gcaagagtaa cctgctctct cgcttcaccc gcaatgagtt caacctggag
      241 agcaagagca ccatcggggt ggagtttgcc acgcgcagta tccatgtgga gggcaagacg
      301 gtcaaagctc agatctggga tacggctgga caggagagat acagagccat cacatcagcg
      361 tattacagag gtgccgtagg agccctgctg gtgtatgaca tcgccaagca tctgacctat
      421 gagaacgcag agcgctggct gaaagagctg caggatcacg ctgacagcaa tattgtcatc
      481 atgttagtag gaaacaaaag cgacttgcgt catctgaggg ctgttcctat ggatgaagct
      541 aaagcatttg cagagaaaca tgggctgtct ttcttggaga cgtcagcgct ggactcttct
      601 aatgttgaac ttgcctttca gactattctc acagaaatct accgtatcgt gtcccagagg
      661 cagatgtcgg ggcgtggaga tgacagcttc tcccccagct ccaaagtggt tcccattacg
      721 gtgcagccca cacaaaattc tggcaagcag agtgcctgct gtcagaataa ctgagctgcc
      781 acagccaact ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg
      841 caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt
      901 aacgcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
      961 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1021 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1081 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     1141 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     1201 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     1261 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     1321 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     1381 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     1441 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     1501 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     1561 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     1621 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     1681 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     1741 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     1801 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     1861 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     1921 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     1981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2161 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2821 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2881 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     2941 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3001 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3061 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3121 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     3181 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     3241 ggccctgcag ctctagagct cgaattctac aggtcactaa taccatctaa gtagttggtt
//
