LOCUS       sequence-223891-        3292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab14l.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab14l
FEATURES             Location/Qualifiers
     source          1..3292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(678..773)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(810..829)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(811..829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(834..850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(834..850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(952..971)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             963..1772
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1030..1049)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1919..2507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2408..2427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2661..2678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2837..2864)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2956..3042)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3091..3113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3105..3122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3106..3122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggcca ctgcacctta caactactcc tacatcttca
       61 agtacatcat cattggtgat atgggtgtag ggaagtcctg cctgctgcat cagttcacag
      121 agaagaaatt catggcggac cgtccgcaca ctatcggcgt ggagttcggc accaggatca
      181 tggaggtgag cggacagaag atcaagctgc agatctggga cacggcgggt caggagcgct
      241 tcagagccgt cacacgcagc tactatagag gagccgctgg agcactgatg gtctacgaca
      301 tcaccaggag aagcacatac aaccacctca gcagctggct gacggacgcc agaaacctca
      361 ccaaccccaa cactgtcatc atactgatag gaaataaagc cgatctggag gcgcagcggg
      421 acgtgacgta cgaggaggcc aagcagtttg cggaggagaa cggtctgctg ttcctggagg
      481 ccagcgctaa aaccggagag aacgtagagg acgctttcct ggaagcagcc aagaagatct
      541 accagaacat tcaggacggc agtctggatc tgaacgcggc agaatccggc gtccagcaca
      601 aaccctcagc gccgcaggga ggaagactga cgagtgaaac acagccgcag cgggaggggt
      661 gtggctgcta attcatccaa ctttattata caaagttggc attataaaaa agcattgctt
      721 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttggagctc
      781 catggtagcg ttaacgcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag
      841 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
      901 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
      961 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     1021 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     1081 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     1141 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     1201 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     1261 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     1321 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     1381 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     1441 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1501 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     1561 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     1621 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     1681 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     1741 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     1801 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     1861 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2041 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2281 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2521 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2581 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2641 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2701 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2761 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     2821 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     2881 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     2941 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3001 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     3061 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     3121 gtcttaagct cgggccctgc agctctagag ctcgaattct acaggtcact aataccatct
     3181 aagtagttgg ttcatagtga ctgcatatgt tgtgttttac agtattatgt agtctgtttt
     3241 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tc
//
