LOCUS       sequence-223892-        3277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab15
FEATURES             Location/Qualifiers
     source          1..3277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(763..858)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(895..914)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(896..914)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(919..935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(919..935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1037..1056)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1048..1857
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1115..1134)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2004..2592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2493..2512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2746..2763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2922..2949)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3041..3127)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3176..3198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3190..3207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3191..3207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcca aacagtacga tgtgctgttc agactcctgc tgctcgggga ctccggtgtc
      181 ggcaaaacct gtttattatg cagattcacg gacaatgaat ttcatccctc tcacatttcc
      241 accatcggtg tcgatttcaa aatgaagacc ttagagatag atggcattaa agtacggata
      301 cagatatggg acacagcggg tcaggagaga tatcagacca tcactaagca gtactacaga
      361 agagcacagg gtatatttct ggtgtatgac atcacaagcg agcgctcgtt ccagcacatc
      421 atgaagtggg ccagtgatgt agatgagtac gcccctgaaa aggtgcagaa gatacttgtt
      481 gggaacaaat ctgatgaaca agagaagaga caagtagcta cagaacaggg agataagctc
      541 gccaaagctt atggaatgga cttttttgag acaagtgcct ttaccaacca caacattaca
      601 gagtctttca cccgactggc tgaattagta ttacaagcca acaagaagga tcttgatttg
      661 ctgggaggat cattgaatga cgagttgaat ctggctgatt tggaggagca ggaaggtttg
      721 tgtaatgtta gcgacgacac tcgaaagtcc tgctggtgct gacaacttta ttatacaaag
      781 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      841 aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat
      901 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
      961 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1021 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1081 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1141 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1201 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1261 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1321 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1381 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1441 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1501 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1561 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1621 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1681 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1741 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1801 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1861 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     1921 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     1981 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2041 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2101 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2161 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2221 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2281 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2341 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2401 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2461 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2521 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2581 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2641 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2701 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2761 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2821 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2881 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     2941 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3001 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3061 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3121 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cctgcagctc tagagctcga
     3241 attctacagg tcactaatac catctaagta gttggtt
//
