LOCUS       sequence-223898-        3259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab1ba.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab1ba
FEATURES             Location/Qualifiers
     source          1..3259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(645..740)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(777..796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(778..796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(801..817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(801..817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(919..938)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             930..1739
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(997..1016)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1886..2474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2375..2394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2628..2645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2804..2831)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2923..3009)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3058..3080
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3072..3089
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3073..3089
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgaatc ctgaatatga ctacctgttc aagttgctcc
       61 tcatcggtga ctctggagtg ggaaagtcgt gtctgttgtt gagatttgct gatgacacat
      121 acactgagag ctatatcagc accatcggcg tggacttcaa gatccgcacc attgaacttg
      181 atggcaaaac cattaagcta cagatatggg atactgcagg gcaagaaaga tttcgaacta
      241 tcacctccag ttactatcga ggggctcatg gaatcattgt ggtttatgat gtgacggatc
      301 aggaatcgta taacaatgtt aaacagtggc ttcaggaaat agaccgctac gcaagcgaga
      361 atgtcaacaa gctgctggtg gggaacaaat gcgacctcac caccaagaaa gtagtggact
      421 acacaacagc caaggaattt gctgactctt tagcaattcc cttccttgag acgagcgcta
      481 agaatgccac aaatgtggag caggccttca tgactatggc tgctgagatc aagaagcgta
      541 tgggccctgg agcgacaacc ggaggagaca agcccaacct gaagatcgag agcactcctg
      601 tgcggcagtc tggcggaggc tgctgttgaa cagcttgagc gtgccaactt tattatacaa
      661 agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      721 tcaaaataaa atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct
      781 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
      841 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      901 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
      961 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1021 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1081 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1141 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1201 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1261 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1321 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1381 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1441 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     1501 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1561 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1621 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1681 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1741 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1801 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     1861 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1921 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1981 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2041 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2101 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2161 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2221 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2281 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2341 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2401 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2461 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2521 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2581 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2641 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2701 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2761 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     2821 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     2881 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     2941 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3001 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     3061 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc
     3121 gaattctaca ggtcactaat accatctaag tagttggttc atagtgactg catatgttgt
     3181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     3241 atatcatttt acgtttctc
//
