LOCUS       sequence-223901-        3310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab20
FEATURES             Location/Qualifiers
     source          1..3310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(696..791)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(828..847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(829..847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(852..868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(852..868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(970..989)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             981..1790
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1048..1067)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2679..2696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2855..2882)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2974..3060)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3109..3131
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3123..3140
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3124..3140
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgaacg ttggaaagac ttctctcctc cacagataca
       61 cggagaggaa gtttaaggac acgataagca ccgtcggagg ggcgtttttc ctcaaacagt
      121 ggggtcccta caacatttcc atatgggata ctgcaggtcg agagcagttt cacggtctgg
      181 gctccatgta ctgccgtggc gcagctgctg tcatcctaac atatgatgtc accaactggc
      241 agagcctggc ggagctggag aaccgcttcc tgtccttgac tgacactgcc aacgcagact
      301 gcatcttcgc ccttgtgggg aacaaagcgg acctgaccga cccgttcgct caagagcctg
      361 acagcgagag tgggagcgac ccgcagcctg tagcctcctg cccgactcct cctgccagcc
      421 ctgtccagca caaacaggtg agcagagagg acgctgtggc tctgtacgcc cgcgttctcc
      481 gatacaaagg cctggacgag aagacctgcc tgcccgccga gaggatgtgc ttcgagacca
      541 gcgctaaaac aggttacaac gtggacgctt tgttcgagac cttatttgat ttagtactgc
      601 catccattct gaagaagagg agcgagggtg aagccactgt agatctggag acgccactag
      661 aggtgagaag gactaaagct ggctgctgtg catagcaact ttattataca aagttggcat
      721 tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      781 aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc tatagtgagt
      841 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
      901 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      961 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     1021 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     1081 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1141 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     1201 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     1261 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     1321 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1381 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1441 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1501 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     1561 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1621 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1681 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1741 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     1801 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     1861 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2761 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     2821 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     2881 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     2941 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3001 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3061 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     3121 gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct cgaattctac
     3181 aggtcactaa taccatctaa gtagttggtt catagtgact gcatatgttg tgttttacag
     3241 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     3301 tacgtttctc
//
