LOCUS       sequence-223903-        3229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab22a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab22a
FEATURES             Location/Qualifiers
     source          1..3229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(615..710)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(747..766)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(748..766)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(771..787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(771..787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(889..908)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             900..1709
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(967..986)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1856..2444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2345..2364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2598..2615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2774..2801)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2893..2979)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3028..3050
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3042..3059
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3043..3059
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(3130..3229,1..24))
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggctacgc taagagagct gaaagtatgc cttcttgggg
       61 acacaggagt tggaaagtca agcattgtgt gtagatttgt ggaggatagt tttgatccaa
      121 acataaatcc aacaattgga gcatcgttta tgacaaagac tgtgcaatac caaaacgagc
      181 ttcacaaatt cctcatctgg gacacagcag gacaagaaag gtttcgtgct ctggctccga
      241 tgtactacag aggctctgct gcggccatca tcgtctatga catcactaaa gaggaatcat
      301 tccagacatt gaagaactgg gtgaaggagc ttcgccagca cggaccaccc aacatagtgg
      361 tggctatcgc tggcaacaag tgcgatctct ccgatgccag agaggtatca gagaaggacg
      421 ccaaagacta cgctgactct attcatgcta tatttatcga aaccagtgcc aaaaatgcca
      481 ttaacatcaa tgaggtcttc acacagataa gtgaaaggat cccggtttta gatgcggagg
      541 gcgggtctgc agtcaagggt tttaaactcc gtcgccaacc atcagtatcg accagagaac
      601 gcacttgctg ctgacaactt tattatacaa agttggcatt ataaaaaagc attgcttatc
      661 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt ggagctccat
      721 ggtagcgtta acgcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg
      781 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      841 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      901 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
      961 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     1021 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1081 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     1141 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     1201 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     1261 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     1321 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     1381 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1441 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1501 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1561 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     1621 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1681 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     1741 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     1801 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1981 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2701 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     2761 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     2821 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     2881 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     2941 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     3001 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     3061 ttaagctcgg gccctgcagc tctagagctc gaattctaca ggtcactaat accatctaag
     3121 tagttggttc atagtgactg catatgttgt gttttacagt attatgtagt ctgtttttta
     3181 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctc
//
