LOCUS       sequence-223906-        3247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab24.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab24
FEATURES             Location/Qualifiers
     source          1..3247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(733..828)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(865..884)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(866..884)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(889..905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(889..905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1007..1026)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1018..1827
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1085..1104)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1974..2562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2463..2482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2716..2733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2892..2919)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3011..3097)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3146..3168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3160..3177
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3161..3177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgactg ccatgcgagt ggacgctaag gttgtcatgc ttggcaaaga aagcgttggt
      181 aaaaccagtc tggtggagag atatgtacat cgtcgctttc ttgtgggacc ttaccaaaat
      241 actatagggg ctgcatttgt tgcaaaagcc ctcaatgttg gtgagaaggt gattacactg
      301 ggaatatggg acaccgctgg gtctgaacgt tatgaagcaa tgagcagaat ttactacaga
      361 ggagctcgtg ctgccattgt ctgctatgac ttgactgaca gcagcagttt tggaagggcc
      421 aggttctggg tgaaggagtt gcagaactgt gaggagcact gcaaaatata tctatgtggt
      481 accaaaagtg acctgattga ggcggaccga agcacgcggc aagtagacta ccatgatgtt
      541 caagactttg cagatgaaat aggtgcacaa catttcgaga cgtccagcaa aacaggaaaa
      601 aatgttgatg aggtgtttca gaaggtggct gaagatttta gtagctgtgc tttagagttc
      661 atgcaagagg agaagggggt ggatctcggg caaaagaagg actcttattt tgacaactgc
      721 tgccataact gacaacttta ttatacaaag ttggcattat aaaaaagcat tgcttatcaa
      781 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg agctccatgg
      841 tagcgttaac gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt
      901 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      961 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1021 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1081 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1141 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1201 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1261 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1321 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1381 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1441 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1501 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1561 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1621 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1681 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1741 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1801 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     1861 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     1921 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2101 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2641 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2701 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     2761 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     2821 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     2881 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     2941 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3001 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3061 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3121 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3181 aagctcgggc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
     3241 gttggtt
//
