LOCUS       sequence-223922-       10355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Foxo1 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Foxo1
FEATURES             Location/Qualifiers
     source          1..10355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2827..2836
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4816..5404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4869..4889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5287..5298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5407..5423)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5408..5424)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5929..6109
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6135..6152)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6141..6365
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6411..6839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6498..6517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6708..6729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6848..6868)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6853..7182
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7033..7168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7095..7114
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7230..7277
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7296..7670
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7800..7921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7837..7856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7891..7910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7970..7986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7970..7986)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7983..8005)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7994..8010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8018..8024,8025..8042,8043..8048))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8063..8084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8201..8218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8372..8960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8452..8471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9131..9922,9923..9991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9754..9773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9992..10096)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10171..10190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgctagcca ccatggccga agcgccccag gtggtggaga ccgacccgga cttcgagccg
     2881 ctgccccggc agcgctcctg tacctggccg ctgcccaggc cggagtttaa ccagtccaac
     2941 tcgaccacct ccagtccggc gccgtcgggc ggcgcggccg ccaaccccga cgccgcggcg
     3001 agcctggcct cggcgtccgc tgtcagcacc gactttatga gcaacctgag cctgctggag
     3061 gagagtgagg acttcgcgcg ggcgccaggc tgcgtggccg tggcggcggc ggctgcggcc
     3121 agcaggggcc tgtgcgggga cttccagggc cccgaggcgg gctgcgtgca cccagcgccg
     3181 ccacagcccc caccgaccgg gccgctgtcg cagcccccac ccgtgcctcc ctccgctgcc
     3241 gccgccgcgg ggccactcgc gggacagccg cgcaagacca gctcgtcgcg ccgcaacgcg
     3301 tggggcaacc tgtcgtacgc cgacctcatc accaaggcca tcgagagctc agccgagaag
     3361 aggctcaccc tgtcgcagat ctacgagtgg atggtgaaga gcgtgcccta cttcaaggat
     3421 aagggcgaca gcaacagctc ggcgggctgg aagaattcaa ttcgccacaa tctgtccctt
     3481 cacagcaagt ttattcgagt gcagaatgaa ggaactggaa agagttcttg gtggatgctc
     3541 aatccagagg gaggcaagag cggaaaatca ccccggagaa gagctgcgtc catggacaac
     3601 aacagtaaat ttgctaagag ccgagggcgg gctgctaaga aaaaagcatc tctccagtct
     3661 gggcaagagg gtcctggaga cagccctggg tctcagtttt ctaagtggcc tgcgagtcct
     3721 gggtcccaca gcaacgatga ctttgataac tggagtacat ttcgtcctcg aaccagctca
     3781 aatgctagta ccatcagtgg gagactttct cccatcatga cagagcagga tgacctggga
     3841 gatggggacg tgcattccct ggtgtatcca ccctctgctg ccaagatggc gtctacgctg
     3901 cccagtctgt ctgaaatcag caatccagaa aacatggaga accttctgga taatctcaac
     3961 cttctctcgt ccccaacatc tttaactgtg tccacccagt cctcgcctgg cagcatgatg
     4021 cagcagacac catgctattc gtttgcaccg ccaaacacca gtctaaattc acccagtcca
     4081 aactactcaa agtacacata cggccaatcc agcatgagcc ctttgcccca gatgcctatg
     4141 cagacacttc aggacagcaa atcaagttac ggaggattga accagtataa ctgtgcccca
     4201 ggactcttga aagagttgtt gacttctgac tctcctcccc acaatgacat tatgtcaccg
     4261 gttgatcccg gagtggccca acccaacagt cgggtcctgg gccaaaatgt aatgatgggc
     4321 cctaattcgg tcatgccagc gtatggcagc caggcatctc ataacaaaat gatgaacccc
     4381 agctcccaca cccaccctgg acatgcacag caaacggctt cggtcaacgg ccgtaccctg
     4441 ccccatgtgg tgaacaccat gcctcacaca tctgccatga accgcttgac ccccgtgaag
     4501 acacctttac aagtgcctct gtcccacccc atgcagatga gtgccctggg cagctactcc
     4561 tcggtgagca gctgcaatgg ctatggtagg atgggtgtcc tccaccagga gaagctccca
     4621 agtgacttgg atggcatgtt tattgagcgc ttggactgtg acatggagtc catcattcgg
     4681 aatgacctca tggatggaga taccttggat tttaactttg ataatgtgtt gcccaaccaa
     4741 agcttcccac acagtgtcaa gactacaaca cacagctggg tgtcaggcta ggaattcgat
     4801 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4861 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4921 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4981 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5041 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5101 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5161 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5221 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5281 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5401 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5461 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5521 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5581 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5641 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     5701 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     5761 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     5821 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     5881 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     5941 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6001 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6061 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     6121 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6181 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6241 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6301 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6361 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6421 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6481 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6541 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6601 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6661 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6721 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6781 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     6841 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6901 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6961 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7021 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7081 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7141 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7201 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     7261 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     7321 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7381 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     7441 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     7501 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     7561 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     7621 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     7681 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7741 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7921 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7981 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8041 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8101 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8161 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10141 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    10201 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
    10261 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
    10321 ttttgcgctg cttcgcgatg tacgggccag atata
//
