LOCUS       sequence-223925-        4411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFPnovo2_unc-54 utr vector for expression in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Hendi A, Mizumoto K
  TITLE     GFPnovo2, a brighter GFP variant for in vivo labeling in C. elegans.
  JOURNAL   MicroPubl Biol. 2018 Sep 6;2018:10.17912/49YB-7K39. doi: 
            10.17912/49YB-7K39.
  PUBMED    32550394
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2018-09-6"; volume: "2018:10.17912/49YB-7K39. doi"; 
            pages: " 10.17912/49YB-7K39"
COMMENT     Sequence Label: pSM-GFPnovo2
FEATURES             Location/Qualifiers
     source          1..4411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(218..246)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(1981..2003)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2103..2122
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2204..2222)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2290..2394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2395..2463,2464..3255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2613..2632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3426..4014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3915..3934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4168..4185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4302..4323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4338..4343,4344..4361,4362..4368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4376..4392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4381..4403
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4400..4411,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4400..4411,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccactgact
       61 gggcgcgcct ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      121 atactaacat aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt
      181 agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      241 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      301 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt
      361 aagtttaaac atatatatac taactaaccc tgattattta aattttcagc caacacttgt
      421 cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca
      481 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      541 agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat
      601 atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      661 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      721 cttcaactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctgt
      781 aagtttaaac atgattttac taactaacta atctgattta aattttcaga acttcaaaat
      841 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      901 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgaccacac aatctgccct
      961 ttcgaaagat cccaacgaaa agagagacca catggtcctt gttgagtttg taacagctgc
     1021 tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt ccaactgagc
     1081 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     1141 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     1201 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     1261 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     1321 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     1381 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     1441 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     1501 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     1561 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     1621 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     1681 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     1741 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     1801 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     1861 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     1921 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1981 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     2041 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     2101 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     2161 atgcgtaagg agaaaatacc gcatcaggcg gccttaatta agggcctcgt gatacgccta
     2221 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4381 agcggataac aatttcacac aggaaacagc t
//
