LOCUS       sequence-223927-       10553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Mecom isoform 1 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Mecom 1
FEATURES             Location/Qualifiers
     source          1..10553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5014..5602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5067..5087)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5485..5496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5605..5621)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5606..5622)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6127..6307
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6333..6350)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6339..6563
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6609..7037
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6696..6715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6906..6927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7046..7066)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7051..7380
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7231..7366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7293..7312
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7428..7475
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7494..7868
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7998..8119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8035..8054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8089..8108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8168..8184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8168..8184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8181..8203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8192..8208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8216..8222,8223..8240,8241..8246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8261..8282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8399..8416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8570..9158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8650..8669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9329..10120,10121..10189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9952..9971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10190..10294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10369..10388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgctagcca ccatgaagag tgaagaggac ccgcacgaac ccatggcgcc tgacatccac
     2881 gaagaacggc agcaccgctg tgaggactgt gaccagctct ttgaatccaa ggcagagcta
     2941 gccgatcacc agaagttccc atgcagcaca cctcactcgg ccttctccat ggtggaggag
     3001 gacttgcaac aaaacctgga gagtgagagc gatctccgag agatccatgg caaccaggac
     3061 tgtaaggaat gtgaccgagt tttccccgat ctgcaaagct tggagaagca catgctgtca
     3121 catactgagg agagggaata caagtgtgat cagtgtccca aggcatttaa ctggaagtcc
     3181 aatttaattc gccaccagat gtcacatgac agtggaaagc actatgagtg tgaaaactgt
     3241 gccaaggttt tcacggaccc tagcaacctt cagcgacaca ttcgatctca gcatgttggt
     3301 gcccgggctc atgcttgccc cgagtgtggt aaaacatttg ccacttcgtc aggcctcaaa
     3361 cagcacaagc acatccacag cagtgtgaag ccctttatct cattctctca atcaatgtac
     3421 ccatttcctg atagagactt gaggtcgtta cctttgaaaa tggagcccca atcaccaagt
     3481 gaagttaaga aactgcagaa gggaagctct gagtcccctt ttgacctcac cactaagaga
     3541 aaggatgaga agcccttgac ttcaggcccc tcgaagcctt caggaacacc agccacaagc
     3601 caagaccagc ccctggatct aagtatgggc agtaggggta gagccagtgg gacaaagttg
     3661 actgagcctc gaaaaaacca tgtgtttggg gaaaagaaag gaagcaacat ggatactagg
     3721 ccatcttcag atggctcctt gcagcatgcc agacccactc ccttcttcat ggaccccatt
     3781 tatagagtag agaaaagaaa gttaactgac ccgcttgaag ctttgaaaga aaaatacttg
     3841 agaccttctc caggattctt gtttcacccg caaatgtcag caattgagaa catggcagaa
     3901 aagctggaaa gcttcagcgc cctcaaacct gaggccagcg agctcctgca gtccgtgccc
     3961 tccatgttca gcttccgagc tcctcccaac accctgccag agaacctgct gcggaagggg
     4021 aaagagcgct acacctgcag gtactgtggc aagatatttc caaggtctgc gaacctaaca
     4081 cggcacttga gaacccacac aggagagcaa ccttacagat gcaaatactg tgatagatca
     4141 ttcagcattt cttccaacct gcagcgacat gtgcgcaaca tccacaacaa ggagaagcca
     4201 tttaagtgtc atttatgtga cagatgtttt ggtcaacaaa ccaatcttga cagacacctg
     4261 aagaaacatg agaacggcaa catgtctggg acggcaacgt cctcgcctca ctcagagcta
     4321 gaaagcgcag gcgcaatcct ggatgacaaa gaagatgcgt actttacaga gatccgcaat
     4381 ttcatcggga acagcaacca tggtagccag tctcctcgga acatggaaga gaggatgaat
     4441 ggcagtcact tcaaggataa aaaggctttg gcaaccagcc aaaattcaga tttattggat
     4501 gatgaagaag tagaagatga ggtgttgttg gatgaggagg atgaagacaa tgatattcct
     4561 ggaaagccca gaaaggagct aggggtgact cgtttagacg aggagatccc ggaggatgac
     4621 tacgaagaag ctggtgccct ggagatgagc tgtaaggcgt ccccggtgag gtataaagag
     4681 gaagactata aatctggcct ttctgctcta gatcacataa ggcacttcac agatagcctc
     4741 aaaatgaggg aaatggaaga gaatcaatac actgacgctg agctgtcctc cattagttct
     4801 tctcatgtgc cagaggagct taaacagacg ttacacagaa agtccaaatc acaggcatat
     4861 gctatgatgt tgtcactgtc tgacaaggat tccctccatc ccacctccca cagttcctcc
     4921 aacgtgtggc acagcatggc aagggctgca gcagaatcca gtgccatcca gtccataagc
     4981 catgtatagg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     5041 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5101 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5161 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5221 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5281 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5341 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5401 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5461 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5521 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5581 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     5641 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5701 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5761 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5821 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5881 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     5941 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     6001 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     6061 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     6121 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6181 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6241 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6301 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6361 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6421 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6481 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6541 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6601 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6901 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6961 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7021 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7081 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7141 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7201 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7261 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     7321 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7381 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     7441 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     7501 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     7561 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     7621 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     7681 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     7741 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     7801 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     7861 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7921 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7981 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8101 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8161 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8221 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8281 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8341 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8401 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8461 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9001 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10321 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    10381 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
    10441 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
    10501 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat ata
//
