LOCUS       sequence-223928-        8786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Mecom isoform 2 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Mecom 2
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3247..3835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3300..3320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3718..3729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3838..3854)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3839..3855)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4360..4540
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4566..4583)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4572..4796
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4842..5270
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4929..4948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5139..5160
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5279..5299)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5284..5613
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5464..5599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5526..5545
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5661..5708
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5727..6101
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6231..6352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6268..6287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6322..6341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6401..6417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6401..6417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6414..6436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6425..6441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6449..6455,6456..6473,6474..6479))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6494..6515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6632..6649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6803..7391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6883..6902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7562..8353,8354..8422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8185..8204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8423..8527)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8602..8621)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgctagcca ccatgagatc caaaggcagg gcaaggaaac tggccacaag taatgagtgt
     2881 gcctatggca actatcctga aatacctttg gaagaaatgc cagatgctga tgcagatggg
     2941 ataaccagtg ttccctccct ccacattcaa gagccatgct ctcctgcgac gtccagtgag
     3001 tcatttactc ctaaggaggg ctcgccatac aaagctccca tctacatccc tgatgacatc
     3061 cctattcctg atgagtttga gcttcgagag tcaactatgc ctggagcagg acttggaata
     3121 tggaccaaaa ggaagattga aataggcgaa aagtttgggc catacatggg agagcagaga
     3181 tcagacctga aagattccag ctatggatgg gagaacaggt aggaattcga tatcaagctt
     3241 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     3841 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     3901 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     3961 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4021 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     4081 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     4141 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     4201 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     4261 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     4321 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     4381 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4441 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4501 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     4561 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4621 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4681 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4741 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4801 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5041 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5161 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5221 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5281 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5341 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5401 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5461 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5521 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5581 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5641 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     5701 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     5761 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     5821 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     5881 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     5941 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     6001 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     6061 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     6121 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6181 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6361 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6421 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6481 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6541 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7201 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7681 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8401 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8521 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     8581 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     8641 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8701 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     8761 gcttcgcgat gtacgggcca gatata
//
