LOCUS       sequence-223929-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Tet-on 3G from the EF1Alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: pHR-EF1Alpha-Tet-on 3G
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          372..1294
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1251..1271
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1390..2133
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             2143..2199
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2275..2863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2328..2348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2746..2757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2866..2882)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2867..2883)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2973..3206
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3231..3249)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3232..3249)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3539..3558)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3772..3791)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3891..3913
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3951..3969)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4037..4141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4142..4210,4211..5002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4360..4379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5173..5761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5662..5681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5915..5932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6002..6022)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6007..6336
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6187..6322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7470..7535
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7665..7685
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8110..8244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8147..8166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8201..8220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8413..9046
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9093..9218
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9715..9947
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10132..10176
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
       61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      181 atcgataagc ttgatatcga attcctgcag ccaattgaac cggtgcctag agaaggtggc
      241 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
      301 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
      361 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
      421 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
      481 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
      541 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      601 tgctttcgat aagtctctag ccatttaaaa tttttgatga cgtgctgcga cgcttttttt
      661 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
      721 gcccgcggcc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      781 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
      841 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
      901 accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg
      961 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     1021 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     1081 cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1141 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     1201 gtaattctcc ttggaatttg gcctttttga gtttggatct tggttcattc tcaagcctca
     1261 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacagggg atctggagct
     1321 ctcgagaatt ctcacgcgtc tgcaggatat caagcttgcg gtaccgcggg cccgggatcc
     1381 gccgccacca tgtctagact ggacaagagc aaagtcataa actctgctct ggaattactc
     1441 aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     1501 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     1561 gagatgctgg acaggcatca tacccactcc tgccccctgg aaggcgagtc atggcaagac
     1621 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     1681 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     1741 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     1801 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     1861 agagagacac ctaccaccga ttctatgccc ccacttctga aacaagcaat tgagctgttc
     1921 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     1981 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     2041 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     2101 cttgacgatt ttgaccttga catgctcccc gggggaagcg gagctactaa cttcagcctg
     2161 ctgaagcagg ctggagacgt ggaggagaac cctggacctg caccaggaag tgcggccgca
     2221 taagactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
     2281 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2341 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2401 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2461 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2521 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2581 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2641 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2701 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2761 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2821 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     2881 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     2941 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3001 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3061 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3121 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3181 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     3241 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     3301 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     3361 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     3421 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     3481 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     3541 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     3601 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     3661 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     3721 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     3781 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3841 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3901 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3961 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4381 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5281 ggtaactggc ttcagcagag cgcagatacc aaatactgtc tttctagtgt agccgtagtt
     5341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5941 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6001 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6061 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6181 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6301 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     6361 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     6421 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     6481 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     6541 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     6601 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     6661 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     6721 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     6781 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     6841 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     6901 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     6961 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     7021 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     7081 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     7141 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     7201 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     7261 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     7321 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     7381 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     7441 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     7501 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     7561 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     7621 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     7681 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     7741 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     7801 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     7861 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     7921 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     7981 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     8041 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     8101 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8161 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8221 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     8281 cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt tactctaaac
     8341 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     8401 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     8461 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     8521 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     8581 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     8641 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     8701 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     8761 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     8821 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     8881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     9061 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     9121 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9181 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9241 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9301 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9361 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9421 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9481 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9541 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9601 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9661 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9721 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9781 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9841 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9901 ggcaagaatc ctggctgtga aagataccta aaggatcaac agctcctggg gatttggggt
     9961 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    10021 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    10081 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    10141 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    10201 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    10261 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    10321 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    10381 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtat
//
