LOCUS       sequence-223938-        8368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA targeting DHC's C-terminal locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK308
FEATURES             Location/Qualifiers
     source          1..8368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          27..254
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      266..275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             275..340
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             347..367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             392..4492
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             392..4492
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4493..4540
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4568..4585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4574..4781
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4790..4930
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4790..4919
                     /label=AAV2 ITR
     rep_origin      5005..5460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5092..5111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5302..5323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5477..5496)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5596..5618
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5656..5674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5742..5846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5847..5915,5916..6707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6065..6084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6878..7466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7367..7386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7528..7776
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7528..7548
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7699..7718
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7798..7873
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        7970..8255
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
       61 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      121 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag aggtaagggt
      181 ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac ctgcctgaaa
      241 tcactttttt tcaggttgga ccggtgccac catggactat aaggaccacg acggagacta
      301 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg
      361 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat
      421 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
      481 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
      541 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
      601 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
      661 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
      721 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
      781 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
      841 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
      901 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
      961 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     1021 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     1081 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     1141 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     1201 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     1261 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     1321 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     1381 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     1441 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     1501 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     1561 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     1621 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     1681 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     1741 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     1801 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     1861 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     1921 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     1981 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     2041 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     2101 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     2161 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     2221 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     2281 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     2341 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     2401 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     2461 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     2521 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     2581 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     2641 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     2701 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     2761 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     2821 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     2881 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     2941 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     3001 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     3061 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     3121 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     3181 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     3241 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     3301 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     3361 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     3421 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     3481 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     3541 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     3601 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     3661 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     3721 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     3781 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     3841 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     3901 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     3961 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     4021 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     4081 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     4141 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     4201 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     4261 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     4321 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     4381 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     4441 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc
     4501 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taagaattcc tagagctcgc
     4561 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4621 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4681 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4741 aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta
     4801 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4861 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4921 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4981 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     5101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5161 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5221 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     5341 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     5401 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     5461 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5521 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5581 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5641 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5701 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6541 aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta
     6601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7021 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7501 ccttttgctg gccttttgct cacatgtgag ggcctatttc ccatgattcc ttcatatttg
     7561 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     7621 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     7681 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     7741 ttggctttat atatcttgtg gaaaggacga aacaccgcct cgcagcttct acgagcggtt
     7801 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     7861 accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc
     7921 gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaggtaccc gttacataac
     7981 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag
     8041 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     8101 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     8161 gtaaatggcc cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc
     8221 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
     8281 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
     8341 tattttgtgc agcgatgggg gcgggggg
//
