LOCUS       sequence-223957-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR G51PEKN mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Liu CT, Hanoian P, French JB, Pringle TH, Hammes-Schiffer S, 
            Benkovic SJ
  TITLE     Functional significance of evolving protein sequence in 
            dihydrofolate reductase from bacteria to humans.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10159-64. doi: 
            10.1073/pnas.1307130110. Epub 2013 Jun 3.
  PUBMED    23733948
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1307130110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-06-18- 18"; volume: "110"; issue: "25"; pages: 
            "10159-64"
COMMENT     Sequence Label: pET22b-ecDHFR-G51PEKN
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             568..585
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(638..656)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      652..699
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgatcag tctgattgcg gcgctagcgg
       61 tagatcgcgt tatcggcatg gaaaacgcca tgccatggaa cctgcctgcc gatctcgcct
      121 ggtttaaacg caacacctta aataaacccg tgattatggg gcgccatacc tgggaatcaa
      181 tcccagaaaa aaacaggcct ttgcccggcc gcaaaaatat tatcctcagc agtcaacccg
      241 ggaccgatga tcgggttacc tgggttaaat cggtcgacga agccatcgcg gcgtgtggtg
      301 acgtaccaga aatcatggtg attggcggcg gacgcgttta tgaacagttc ttgccaaaag
      361 cgcaaaaact gtatctgacg catatcgatg cagaagtgga aggcgacacc cattttccgg
      421 attacgagcc ggatgactgg gaatcggtat tcagcgaatt ccacgatgct gatgcgcaga
      481 actcgcatag ctattgtttc gaaatcctcg agcgtcgtta aggatccgaa ttcgagctcc
      541 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      601 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      661 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      721 ggattggcga atgggacgcg ccctgtagcg gcg
//
