LOCUS       sequence-223959-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR N23PP and L28F mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Liu CT, Hanoian P, French JB, Pringle TH, Hammes-Schiffer S, 
            Benkovic SJ
  TITLE     Functional significance of evolving protein sequence in 
            dihydrofolate reductase from bacteria to humans.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10159-64. doi: 
            10.1073/pnas.1307130110. Epub 2013 Jun 3.
  PUBMED    23733948
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1307130110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-06-18- 18"; volume: "110"; issue: "25"; pages: 
            "10159-64"
COMMENT     Sequence Label: pET27b-ecDHFR-N23PP/L28F
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             561..593
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             600..617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(670..688)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      684..731
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agaaggagat atacatatga tcagtctgat tgcggcgcta gcggtagatc gcgttatcgg
       61 catggaaaac gccatgccat ggccgccgct gcctgccgat ttcgcctggt ttaaacgcaa
      121 caccttaaat aaacccgtga ttatggggcg ccatacctgg gaatcaatcg gtaggccttt
      181 gcccggccgc aaaaatatta tcctcagcag tcaacccggg accgatgatc gggttacctg
      241 ggttaaatcg gtcgacgaag ccatcgcggc gtgtggtgac gtaccagaaa tcatggtgat
      301 tggcggcgga cgcgtttatg aacagttctt gccaaaagcg caaaaactgt atctgacgca
      361 tatcgatgca gaagtggaag gcgacaccca ttttccggat tacgagccgg atgactggga
      421 atcggtattc agcgaattcc acgatgctga tgcgcagaac tcgcatagct attgtttcga
      481 aatcctcgag cgtcgttaag gatccgaatt cgagctccgt cgacaagctt gcggccgcac
      541 tcgagatcaa acgggctagc cagccagaac tcgccccgga agaccccgag gatgtcgagc
      601 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      661 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      721 ggggtttttt gctgaaagga gg
//
