LOCUS       sequence-223962-        3307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab27a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab27a
FEATURES             Location/Qualifiers
     source          1..3307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(693..788)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(825..844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(849..865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(849..865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(967..986)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             978..1787
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1045..1064)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1934..2522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2423..2442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2676..2693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2852..2879)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2971..3057)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3106..3128
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3120..3137
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3121..3137
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgtccg atggggacta tgattacctc atcaagttcc
       61 tcgctctggg ggactctggg gtgggaaaga ccagcttcct atatcagtac acagacagca
      121 aattcaactc caaattcata accacagtgg gaatagactt cagagaaaaa cgagtggtgt
      181 ataaatccag tggtcctgat ggcacagcag gcagaggcca gaggatccac atccagcttt
      241 gggatacggc cggacaggag aggtttcgaa gtttgaccac tgctttcttc agagatgcga
      301 tgggctttct gctgctcttt gacctcacca acgagcagag cttcctgaat gtgcgcaact
      361 ggatgagtca gctgcagaca catgcgtact gtgagaatcc tgatatcgtc ttgtgtggaa
      421 ataaatcaga cctggaggag cagcgtgccg tcaaagcgga gaacgcaaga gaactggcag
      481 agaaatacgg cgtgccatac ttcgagacga gcgcggcgaa cggtgagaat gtgagtcgtg
      541 cggtggaggt tctgctggat ctgatcatga agcgcatgga gagatgtgtg gataaatcct
      601 ggatccccga cgggacagtc cgcagcaacg gccacagcac cgccgacctg acagaaccac
      661 acgaccccga gcagagcaag tgtgcatgct aacaacttta ttatacaaag ttggcattat
      721 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      781 cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat agtgagtcgt
      841 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      901 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      961 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1021 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1081 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1141 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1201 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1261 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     1321 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1381 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     1441 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1501 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     1561 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1621 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1681 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1741 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     1801 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     1861 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     1921 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1981 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2041 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2101 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2161 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2221 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2281 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2341 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2401 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2461 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2521 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2581 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2641 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2701 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2761 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     2821 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     2881 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     2941 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     3001 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     3061 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     3121 gtaaaacgac ggccagtctt aagctcgggc cctgcagctc tagagctcga attctacagg
     3181 tcactaatac catctaagta gttggttcat agtgactgca tatgttgtgt tttacagtat
     3241 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     3301 gtttctc
//
