LOCUS       sequence-223964-        3277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab2a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab2a
FEATURES             Location/Qualifiers
     source          1..3277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(663..758)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(795..814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(796..814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(819..835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(819..835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(937..956)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             948..1757
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1015..1034)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1904..2492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2393..2412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2646..2663
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2822..2849)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2941..3027)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3076..3098
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3090..3107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3091..3107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggcgt acgcgtatct cttcaagtac atcatcatcg
       61 gagacacggg tgtgggaaag tcgtgcctat tattacagtt cacagataag cgctttcagc
      121 cggtgcacga tctcactatt ggtgttgaat ttggagcgcg aatgatcact atagatggca
      181 aacagataaa acttcagatc tgggatacgg ctggacagga gtccttccgc tcaatcacca
      241 ggtcttacta cagaggtgca gctggagctc tgctagttta tgacatcaca agaagagaca
      301 cttttaatca cttgacaacc tggttagaag atgctcgtca acattccaac tctaacatgg
      361 tcatcatgct catcggaaat aaaagtgatt tggagtcacg gagagaggtg aagaaagagg
      421 aaggagaggc ctttgccaga gaacatggct tgatcttcat ggagacttct gcaaagaccg
      481 catcaaacgt agaagaggca tttatcaaca cagctaagga gatttatgaa aagatccagg
      541 agggagtctt tgatataaac aacgaggcta acggcattaa gattggaccc cagcatgctg
      601 ccaccaactc cacgatgggc ggcagtcagg gaggacagca agccggaggg ggctgttgct
      661 gacaacttta ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
      721 cgaacaggtc actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac
      781 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
      841 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      901 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
      961 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     1021 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     1081 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     1141 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     1201 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     1261 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     1321 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     1381 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     1441 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     1501 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     1561 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     1621 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     1681 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     1741 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     1801 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     1861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2521 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2581 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2641 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2701 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2761 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     2821 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     2881 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     2941 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     3001 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     3061 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     3121 cctgcagctc tagagctcga attctacagg tcactaatac catctaagta gttggttcat
     3181 agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
     3241 tttaatatat tgatatttat atcattttac gtttctc
//
