LOCUS       sequence-223968-        3349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab33a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab33a
FEATURES             Location/Qualifiers
     source          1..3349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(835..930)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(967..986)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(968..986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(991..1007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(991..1007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1109..1128)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1120..1929
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1187..1206)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2076..2664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2565..2584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2818..2835
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2994..3021)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3113..3199)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3248..3270
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3262..3279
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3263..3279
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcaa atgaattctc agaaaacaga gacggaactg ccaactcacg ccatgccaac
      181 ctcacgtcgt cactagacct gagcacgtct ctggatcaca gcgtgcagac gcggattttt
      241 aaaatcattg tcatcgggga ctcaaatgtg ggaaagacct gtttaacctt ccgcttcacc
      301 ggcggagcct tcccgtgcaa aactgaagcc accatcggcg tggatttcag ggagaaagcg
      361 gtggagatcg agggcgagaa aataaaggta caagtatggg acacagcagg ccaggagcgc
      421 tttcgaaaga gcatggttga gcactactac cgcaatgtgc atgctgttgt ttttgtgtac
      481 gacgtcacta agatggcctc attccaaaac ctaaaaacct ggattcagga gtgcaacggt
      541 cacggggtgt catcggcagt gcctcgtgtt ttggtgggca acaaatgtga tctagttgac
      601 cagatccagg tgccttccaa cactgccctc aagtttgccg atgcctataa catgctactg
      661 tttgaaacat cagccaaaga tcccaaggag agccagaacg tggactccat tttcatgtgc
      721 ctggcatgtc gtctaaaagc ccaaaagtcc ctgatttaca gagatgtgga gagagaagat
      781 ggcagagtaa ggctgacgca tcaaccagac cccaagagta actgcccgtg ctgacaactt
      841 tattatacaa agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
      901 tcactatcag tcaaaataaa atcattattt ggagctccat ggtagcgtta acgcggccgc
      961 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1021 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1081 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1141 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1201 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1261 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1321 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1381 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1441 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1501 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1561 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1621 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1681 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1741 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1801 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1861 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1921 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     1981 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2221 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2821 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2881 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2941 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3001 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3061 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3121 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3181 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3241 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc
     3301 tctagagctc gaattctaca ggtcactaat accatctaag tagttggtt
//
