LOCUS       sequence-223969-        3367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab33ba.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab33ba
FEATURES             Location/Qualifiers
     source          1..3367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(753..848)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(885..904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(886..904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(909..925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(909..925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1027..1046)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1038..1847
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1105..1124)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2736..2753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2912..2939)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3031..3117)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3166..3188
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3180..3197
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3181..3197
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggcag atatcgagtc ctcttttgag ttcagctctt
       61 ctctgaccag ctcgtcactg cctcctcctc ggacacgcat ctttaaaatc atcgtgattg
      121 gagactcagg ggttggcaag acttgcctca cctatagatt ctgtgcaggc aagtttccag
      181 acaagactga ggccactatt ggggtggatt tccgagagaa ggttattgag atagatggcg
      241 agaaaatcaa ggtccagttg tgggacacgg caggccagga gcgtttccgt aagagcatgg
      301 tgcagcacta ctaccgcaac gtccatgctg tggtgtttgt ctatgacgtc accaatgccg
      361 ccagcttccg aagcctccca gcgtggattg aagagtgtcg ccaacatgca ctcggtcagg
      421 aggtgccaag aatccttgtc ggcaacaaat gcgacctacg tcacgccgct caagtaagca
      481 cagatgttgc acagcagttt gcagatacac actcaatgcc tctttttgag acctcggcca
      541 aaaaccccta cgggaatgaa gacggcactc agaataatag tgaccacgtg gaggccattt
      601 ttatgacagt ggcgcacaag ctgaaatccc aaaagccgct ggttttaagc cagccacctt
      661 gcggatatgg agatactgtc actcttagga ggcaagacca ggaggacggg ggaaactggg
      721 gctgcggttg ttggagaagc taatgaagtg cacaacttta ttatacaaag ttggcattat
      781 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      841 cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat agtgagtcgt
      901 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      961 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1021 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1081 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1141 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1201 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1261 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1321 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     1381 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1441 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     1501 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1561 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     1621 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1681 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1741 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1801 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     1861 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     1921 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     2881 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     2941 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     3001 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     3061 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     3121 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     3181 gtaaaacgac ggccagtctt aagctcgggc cctgcagctc tagagctcga attctacagg
     3241 tcactaatac catctaagta gttggttcat agtgactgca tatgttgtgt tttacagtat
     3301 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     3361 gtttctc
//
