LOCUS       sequence-223970-        3418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab34a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab34a
FEATURES             Location/Qualifiers
     source          1..3418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(904..999)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1036..1055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1037..1055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1060..1076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1060..1076)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1178..1197)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1189..1998
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1256..1275)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2145..2733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2634..2653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2887..2904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3063..3090)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3182..3268)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3317..3339
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3331..3348
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3332..3348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgagtg tactgccccc tgtacgaaaa gaccgcataa tcggacagct tcctaagtgc
      181 ttcaacagag gcgctgcttt gaacacagag aatgaatttc acagcaaggt gaagacggcc
      241 tgtcagcagc agaggacagg aactgtgggg tttaagatct ccaaggttat tgtagttggt
      301 gatctggcgg tggggaaaac ctgtttaatt aatagatttt gtaaagatgt ctttgacaaa
      361 aattacaaag ctaccattgg tgtggacttt gaaatggaga ggtttgaagt tctcggagtc
      421 cccttcagtc tgcaactatg ggacactgca ggacaggaga ggtttaaatg cattgcctca
      481 acatactaca gaggagctca ggctgtaatc attgtgtttg acctgactga tgtggcttca
      541 ctggagcaca ccagacaatg gcttgaagac gctatgaaag agaatgatcc taccagtgta
      601 ttactgttct tagtgggaac aaagaaagat ctgagttctc ctgcgcagta ttcactaatt
      661 gagcaggacg caattaaaat agcggatcag atgaaagcgg agtactgggc tttgtctgcc
      721 ttgtctgggg agaatgtgag agactttttc ttcagagtcg catctttaac ctttgaggcc
      781 aatgtactgg cagaactgga gaaaagtgga tcaaggcgta ttggggaagt tgttcaaatc
      841 agcagcaact ctaataacct gtacgcaacg tccaagaaga aacagagcaa ctgttgtcag
      901 tagcaacttt attatacaaa gttggcatta taaaaaagca ttgcttatca atttgttgca
      961 acgaacaggt cactatcagt caaaataaaa tcattatttg gagctccatg gtagcgttaa
     1021 cgcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1081 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1141 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     1201 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     1261 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     1321 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     1381 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     1441 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     1501 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     1561 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     1621 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     1681 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     1741 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     1801 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     1861 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     1921 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     1981 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     2041 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     2101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3001 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3061 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     3121 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     3181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     3241 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     3301 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     3361 ccctgcagct ctagagctcg aattctacag gtcactaata ccatctaagt agttggtt
//
