LOCUS       sequence-223971-        3408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab34b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab34b
FEATURES             Location/Qualifiers
     source          1..3408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(794..889)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(926..945)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(927..945)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(950..966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(950..966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1068..1087)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1079..1888
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1146..1165)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2035..2623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2524..2543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2777..2794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2953..2980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3072..3158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3207..3229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3221..3238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3222..3238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgatga attctttacc ccctgtgaaa ggagaccgga
       61 tcatctgtga actccctttg tattttacaa gagatgctgc agtgcacacc aaagatggct
      121 tcaatattac tgttagagag gcttgtcaag ggcagaaaac aggattaaat gtagccaaag
      181 tcattgtcgt tggtgatgtt ggggtcggga agacctgttt aataaacaga ttctgcaaag
      241 actcatttga cagaacgtac aaggcaacta ttggagtgga ttttgagatg gagagatttg
      301 aagttctcgg ggtacccttc agtttgcagc tgtgggacac agcaggtcag gaaagatttc
      361 gatgcattgc ctccacatac tacagaggag cgcaagccat catcgttgtg tttgacctga
      421 gcaactatga gtctctggat cacgcgagag aatggcttga agatgccatg agggacaatg
      481 acccgtccag tgtcctgctc ttcctcgtgg gaaccaagaa ggatcttagt catccagatc
      541 tgttggccca aatggagcag gaggccattc gattgtctga ggagatcaga gcagagtact
      601 gggcagtttc tgctttgtca ggggcgagtg tgagagattt cttcctgcgg gttgcttcgc
      661 ttacatttga ggccactgtt ttatctgagc tggagaagaa caattctaga cgagccggtg
      721 atatcgtcaa aatctccaat aattgtgaca acaattttaa gaagaggcaa tccagctgct
      781 gccattgact gaacaacttt attatacaaa gttggcatta taaaaaagca ttgcttatca
      841 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gagctccatg
      901 gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt
      961 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1021 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1081 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1141 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1201 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1261 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1321 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1381 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1441 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1501 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1561 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1621 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1681 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     1741 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     1801 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     1861 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     1921 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     1981 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2161 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2701 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2761 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2821 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2881 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
     2941 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
     3001 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
     3061 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     3121 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     3181 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     3241 taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata ccatctaagt
     3301 agttggttca tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat
     3361 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctc
//
