LOCUS       sequence-223974-        3427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab36.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab36
FEATURES             Location/Qualifiers
     source          1..3427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(913..1008)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1045..1064)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1046..1064)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1069..1085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1069..1085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1187..1206)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1198..2007
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1265..1284)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2154..2742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2643..2662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2896..2913
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3072..3099)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3191..3277)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3326..3348
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3340..3357
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3341..3357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgcagt tcagtccacc tgtcagcaga gacaggatca tatccgcatt tccaaagtgt
      181 tataatccac aagcttgcct gcaaatgaag gatgactgga atgtagaagc caaggtggct
      241 tgccaagatc ggacgactcg tctgcaaggg tatgacaggt ttaagatgtc aaaggcagta
      301 gtggttggag atctcaatgt gggaaagaca tgcttaataa acaggttctg taaagacgtg
      361 tttgagagag actataaggc tactataggt gttgattttg agatcgagag atttgagatg
      421 tctgggctgc ctttttctct tcaaatatgg gacaccgctg gtcaagaaaa gtttaaatgc
      481 attgcatcag catattacag aggagctcaa gttattatca ctgtctttga catggcggat
      541 attaagacct tggaaaacac aaaacagtgg ttgttagagg cggtgcggga aaatgaacca
      601 gactcctgtt ttgttttctt ggttggcaca aaaagagacc tcctgtctac agaagagtgc
      661 cagaggaccg agagggacgc cgtaaagatt gcagcagaaa tgaatgcaga attctggtca
      721 gtgtcctcaa aaacaggtga aaacgtccaa gagtttttct ttcgtgtggc tgctctggct
      781 tttgaagatg ctgtcatgaa ggacctggag tctgggacca ccagcagcac tcagatcgga
      841 gatggcagta ttctcaatga taaaacactg gaagtcacgc aggagaagcc aaagaagaag
      901 agctgttgct gacaacttta ttatacaaag ttggcattat aaaaaagcat tgcttatcaa
      961 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg agctccatgg
     1021 tagcgttaac gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt
     1081 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1141 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1201 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1261 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1321 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1381 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1441 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1501 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1561 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1621 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1681 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1741 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1801 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1861 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1921 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1981 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2041 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2101 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2161 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2221 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2281 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2341 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2401 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2461 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2521 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2581 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2641 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2701 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2761 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2821 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2881 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     2941 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3001 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3061 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3121 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3181 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3241 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3301 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3361 aagctcgggc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
     3421 gttggtt
//
