LOCUS       sequence-223981-        3301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab3aa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab3aa
FEATURES             Location/Qualifiers
     source          1..3301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(787..882)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(919..938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(920..938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(943..959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(943..959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1061..1080)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1072..1881
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1139..1158)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2028..2616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2517..2536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2770..2787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2946..2973)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3065..3151)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3200..3222
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3214..3231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3215..3231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggctt cagcgaatga tgctcggtat ggacaaaaag actctgcaga acagaacttt
      181 gactacatgt ttaaggtcct gatcattggt aacagcagtg ttggcaaaac tagtttcctg
      241 ttccgttatg ctgatgactc cttcacacct gcttttgtca gcactgtcgg catagacttc
      301 aaagtcaaga ccatctatag gaacgacaag aggataaagc tccaaatatg ggacactgct
      361 gggcaggaga ggtacaggac catcaccaca gcttactatc gcggagcaat gggcttcatt
      421 cttatgtatg acatcaccaa cgaggattct tttaatgccg ttcaggactg gtccacacag
      481 attaagacgt attcatggga caacgctcaa gtgctgctgg tgggaaataa gtgtgatatg
      541 gaagatgaga gagttgttgc tggacagaga ggcagacaat tgtctgaaca gctaggcttt
      601 gagttttttg aagccagtgc caaggacaat ataaacgtca agcagacatt tgagcgtctg
      661 gtggatgtaa tctgtgagag aatgtcagag aacctggagt cagcagatcc atccatgact
      721 ggaaacaagc tgggtcccga gctctcagag caaccgcagc gctcacacaa ggactgcgct
      781 tgctaacaac tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt
      841 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt
      901 taacgcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
      961 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1021 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1081 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1141 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1201 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1261 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1321 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1381 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1441 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1501 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1561 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1621 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1681 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1741 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1801 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     1861 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     1921 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     1981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2521 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2581 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2641 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2701 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2761 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2821 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     2881 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     2941 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3001 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3061 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3121 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3181 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3241 gggccctgca gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt
     3301 t
//
