LOCUS       sequence-223982-        3314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab3ab.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab3ab
FEATURES             Location/Qualifiers
     source          1..3314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(700..795)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(832..851)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(833..851)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(856..872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(856..872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(974..993)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             985..1794
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1052..1071)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1941..2529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2430..2449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2683..2700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2859..2886)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2978..3064)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3113..3135
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3127..3144
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3128..3144
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggctt cagctacaga caaccgttat ggacaaaagg
       61 agtcttctga ccagaacttt gattacatgt tcaaaatcct aatcatcggg aatagcagcg
      121 ttgggaagac cagctttctg tttcgctatg cagatgactc cttcacgcca gcgttcgtaa
      181 gcaccgtcgg cattgacttc aaggtgaaga ccatctacag gaacgacaag aggataaagc
      241 tgcaaatctg ggacacagcg gggcaggagc gttacagaac catcaccacc gcttattacc
      301 gtggagccat gggattcatc ctcatgtacg acattaccaa tgaggagtct ttcgctgcag
      361 tgcaggactg gtccacacag attaagacat actcatggga taacgctcag gtgcttctag
      421 taggcaacaa gtgtgacatg gaggatgaga gggtcgtggc ttctgaaagg ggcagacagc
      481 tctctgagca tcttggtttt gaatatttcg aagccagtgc caaggacaac attaatgtta
      541 aacaaacctt cgagcggttg gtggacataa tctgcgagaa gatgtcagag agcctggatg
      601 ctgccgatcc tgcagtgacg ggagccaaac aagggccgca gctcacagag cagcccgccg
      661 ccccgcaccg ggactgcgcc tgctgaaaat cccacagctc aactttatta tacaaagttg
      721 gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      781 ataaaatcat tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt
      841 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
      901 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      961 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1021 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1081 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1141 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1201 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1261 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1321 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1381 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1441 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1501 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1561 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1621 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1681 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     1741 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     1801 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     1861 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     1921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2701 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2761 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     2821 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     2881 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     2941 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3001 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3061 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     3121 cgacgttgta aaacgacggc cagtcttaag ctcgggccct gcagctctag agctcgaatt
     3181 ctacaggtca ctaataccat ctaagtagtt ggttcatagt gactgcatat gttgtgtttt
     3241 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     3301 attttacgtt tctc
//
