LOCUS       sequence-223986-        3502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab40b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab40b
FEATURES             Location/Qualifiers
     source          1..3502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(888..983)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1020..1039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1021..1039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1044..1060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1044..1060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1162..1181)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1173..1982
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1240..1259)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2129..2717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2618..2637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2871..2888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3047..3074)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3166..3252)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3301..3323
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3315..3332
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3316..3332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgggtc atcgggctga tgtgaagatg aaccacagga
       61 gcagcccagc aaaagcttat gattttcttc tgaaattcct actggttgga gacagtgatg
      121 tggggaaagg ggaaatactg gccagtcttc aggatgggtc cagtgagtca ccctatggat
      181 acaacatggg aatcgactac aagactacta ccattctcct ggatggacgc agggttaagc
      241 ttcaactgtg ggacacctca ggacaaggca gattctgtac catattccgc tcctattcca
      301 gaggagcaca gggagtcatc cttgtttatg acatcacaaa ccggtggtct ttcgacggca
      361 tcgacagatg gattaaagag attgatgagc atgcaccagg tgtccccaag atcctggtgg
      421 gcaacaggct tcatctggcc tataagcgtc aggtgaccac cgaacatgcg caggcctttg
      481 cagagcgatt gggcgtcacg ttttttgagg tcagccctct ctgtaacttc aacatcaccg
      541 agtccttcac tgaactggcc cgcattgtgc tcatgaggca cggcatggag cgactctgga
      601 gacccaataa agtgttgagt ctgcaggacc tgtgctgccg ttctatcgtt tcctgcactc
      661 cagtgcatct cgtggacaaa cttccccttc ctgttgcctt aaagagtcat ctgaagtcct
      721 tttccatggc taacggcctg gtcaacacca ggatgatgca cggccgttcc tattcggtca
      781 tacctagcag tgctaaaaaa cggaacggaa ccaaaaaatc caaaatcatc tatcctccac
      841 tgagcccatc ccagcactgc gcacggacaa gctgcaagat ttcatagcaa ctttattata
      901 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      961 cagtcaaaat aaaatcatta tttggagctc catggtagcg ttaacgcggc cgcgatatcc
     1021 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1081 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1141 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     1201 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1261 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1321 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1381 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1441 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     1501 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1561 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     1621 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     1681 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     1741 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     1801 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1861 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1921 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1981 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2041 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2101 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2161 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2221 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2281 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2341 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2401 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2461 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2521 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2581 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2641 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2701 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2761 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2821 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2881 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2941 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3001 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3061 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3121 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3181 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3241 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     3301 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccctgc agctctagag
     3361 ctcgaattct acaggtcact aataccatct aagtagttgg ttcatagtga ctgcatatgt
     3421 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     3481 tttatatcat tttacgtttc tc
//
