LOCUS       sequence-223988-        3489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab40c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab40c
FEATURES             Location/Qualifiers
     source          1..3489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(875..970)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1007..1026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1008..1026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1031..1047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1031..1047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1149..1168)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1160..1969
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1227..1246)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2116..2704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2605..2624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2858..2875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3034..3061)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3153..3239)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgggca cccagggaag ccctgtgaag agctacgact
       61 acctgctcaa gttcctcctg gtcggagaca gcgacgtggg caagggagag atcctggata
      121 gccttcaaga cggatctgct gagtctccat atgcctacag cagcggaatc gactacaaga
      181 caaccacgat cctgttggat ggacggagag ttaaactaga gctttgggac acttcgggac
      241 aaggcaggtt ttgcaccatt ttcaggtcgt actccagggg agcgcagggg atcttgttgg
      301 tgtatgacat caccaaccgc tggtcatttg atgggataga ccgctggatc agggagattg
      361 atgagcatgc acctggagtg ccccgtattc tagtgggtaa ccggctacac ctggcgttca
      421 agcgacaggt tcccacagaa caggcacggg catacgcaga aaagaatggc atgacatttt
      481 ttgaagtgag tcctctctgc aacttcaacg tcatcgagtc ctttacagag ctctcgcgca
      541 tcgtcctgat gaggcatggg atggagaagt tctggaggcc caacagagta ttcagtctgc
      601 aggacttgtg ctgccgtgcc attgtttcct gcacccctgt gcacctaatc gataagctgc
      661 cacttcctgt ggccatcaag agccatttga agtcattctc catggcgaac ggcatgaatg
      721 ctgtcatgat gcacggccgc tcgtattcgc tggccaatgc cgccggaggc agcaaaggta
      781 acagtcttaa acgctccaaa tccatccggc cgccacaaag tccaccacag aactgcacca
      841 ggaacaactg taagatctcc tagcctccag gcaccaactt tattatacaa agttggcatt
      901 ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      961 atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct atagtgagtc
     1021 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1081 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     1141 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     1201 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1261 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1321 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1381 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1441 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     1501 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1561 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1621 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1681 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     1741 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1801 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1861 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     1921 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     1981 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     2041 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     2101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2821 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2881 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2941 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
     3001 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
     3061 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     3121 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     3181 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
     3241 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc gaattctaca
     3361 ggtcactaat accatctaag tagttggttc atagtgactg catatgttgt gttttacagt
     3421 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3481 acgtttctc
//
