LOCUS       sequence-223993-        3300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab4a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab4a
FEATURES             Location/Qualifiers
     source          1..3300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(686..781)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(818..837)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(819..837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(842..858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(842..858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(960..979)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             971..1780
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1038..1057)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1927..2515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2416..2435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2669..2686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2845..2872)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2964..3050)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3099..3121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3113..3130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3114..3130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgtcag agacatacga tttcttgttt aagtttcttg
       61 tgattgggaa tgctggaaca ggaaaatcct gtcttcttca tcagttcata gaaaaaagat
      121 tcaaagatga ctcaaatcac actattgggg ttgaatttgg ttcgaagata atcagcgtgg
      181 tcaataagtt tgtaaagctc cagatctggg acacagcagg acaggagcgt ttcaggtctg
      241 taactaggag ttattacaga ggcgcagcag gggctcttct agtctatgac atcactagtc
      301 gagaaacata caacgctcta accaactggc tgacagatgc ccggatgttg gccagtcaga
      361 acatagtgat catactatgt ggaaacaaga aggatctgga tgctgaccgc gaggtgacct
      421 ttctggaggc ttcacgcttc gctcaggaga atgagcttat gtttttggag accagtgctc
      481 tgacagggga gaatgtggag gaagctttcg tccagtgtgc tcgaaaaatc cttaacaaaa
      541 ttgaatcagg agagctggac ccagagcgga tgggttcagg aatccagtat ggagacgcgg
      601 ctcttcgaca gctgcgttca cccagacggg cacaagccga gagcatccag gagtgtggct
      661 gttaacacac gtccctctga acagacaact ttattataca aagttggcat tataaaaaag
      721 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      781 tggagctcca tggtagcgtt aacgcggccg cgatatcccc tatagtgagt cgtattacat
      841 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      901 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      961 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     1021 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1081 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1141 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     1201 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1261 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1321 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1381 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1441 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     1501 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     1561 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     1621 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     1681 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     1741 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     1801 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     1861 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2581 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2641 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2701 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2761 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
     2821 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
     2881 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     2941 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     3001 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
     3061 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
     3121 gacggccagt cttaagctcg ggccctgcag ctctagagct cgaattctac aggtcactaa
     3181 taccatctaa gtagttggtt catagtgact gcatatgttg tgttttacag tattatgtag
     3241 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
//
