LOCUS       sequence-223998-        9215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Let-7d-5p using miR-451 backbone to enable DICER 
            independent expression along with orange fluorescent protein (OFP) 
            to track miRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Baer C, Squadrito ML, Laoui D, Thompson D, Hansen SK, Kiialainen A, 
            Hoves S, Ries CH, Ooi CH, De Palma M
  TITLE     Suppression of microRNA activity amplifies IFN-gamma-induced 
            macrophage activation and promotes anti-tumour immunity.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):790-802. doi: 10.1038/ncb3371. Epub 
            2016 Jun 13.
  PUBMED    27295554
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3371"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "790-802"
COMMENT     Sequence Label: LV-Let7d-5p
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2292..2671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2672..2875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2826..2846
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2890..3070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3117..3242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3735..3968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4153..4197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4464..4581
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4716..5123
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     intron          6153..6356
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     6313..6333
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6532..7242
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7258..7274)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7259..7275)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7277..7865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7330..7350)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7748..7759)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7952..8185
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8257..8378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8294..8313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8348..8367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8418..8553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8573..8592)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8574..8592)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8602..8619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8602..8618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8611..8633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8760..9188
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8847..8866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9057..9078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttggcc
     2221 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     2281 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2341 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2401 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2461 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2521 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2581 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2641 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2701 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2761 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2821 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2881 agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2941 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3001 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3061 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     3121 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     3181 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     3241 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3301 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3361 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3421 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3481 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3541 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     3601 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3661 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3721 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3781 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3841 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3901 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3961 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     4021 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     4081 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     4141 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     4201 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     4261 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4321 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     4381 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     4441 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     4501 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4561 attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgatcgaga agcttgataa
     4621 ttcctgcagc cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     4681 aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga
     4741 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
     4801 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
     4861 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
     4921 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
     4981 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
     5041 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc
     5101 tccgacagac tgagtcgccc gggggggatc cacgttcttt ttcgcaacgg gtttgccgcc
     5161 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     5221 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     5281 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     5341 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     5401 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     5461 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     5521 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     5581 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     5641 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     5701 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     5761 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     5821 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     5881 ccgtccaggc acctcgatta gttctcgacg tttgcagcag agatgcagaa gtacacgggc
     5941 tcactgctcg gcctaatcaa gcctgctgac agctgtggca cttgggaatg gcgaggagag
     6001 gtagtaggtt gcatagttat gcaacctact acctcatctt gctgctccca caaactgtgc
     6061 caagaagagc tcatgaccct ggagcagact gctggaagaa aaggacaccc aggctgacaa
     6121 ggcgcgttag gctagctacg tttaaactat agtcgagctt ttggagtacg tcgtctttag
     6181 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     6241 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     6301 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     6361 cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc gggctgcagg aattcgggcc
     6421 gcggccagct ccggcgggca gggggggcgc tggagcgcag cgcagcgcag ccccatcagt
     6481 ccgcaaagcg gaccgagctg gaagtcgagc atccatcaca ctggcggccg catggtgagc
     6541 aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcgcatg
     6601 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     6661 gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     6721 gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca ccccgccgac
     6781 atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     6841 tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     6901 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag
     6961 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg tgccctgaag
     7021 ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc cgaggtcaag
     7081 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag
     7141 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     7201 ggccgccact ccaccggcgg catggacgag ctgtacaagt aagaattcga agcttatcga
     7261 taccgtcgac ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7321 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7381 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7441 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7501 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7561 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7621 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7681 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7741 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7801 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7861 cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     7921 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7981 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8041 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8101 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8161 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8221 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8401 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8461 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8521 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     8581 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8641 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8701 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     8761 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8821 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8881 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8941 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     9001 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9061 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9121 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9181 cgcgaatttt aacaaaatat taacgcttac aattt
//
