LOCUS       sequence-224015-       11283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrin B1 overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Er EE, Valiente M, Ganesh K, Zou Y, Agrawal S, Hu J, Griscom B, 
            Rosenblum M, Boire A, Brogi E, Giancotti FG, Schachner M, Malladi S,
            Massague J
  TITLE     Pericyte-like spreading by disseminated cancer cells activates YAP 
            and MRTF for metastatic colonization.
  JOURNAL   Nat Cell Biol. 2018 Aug;20(8):966-978. doi: 
            10.1038/s41556-018-0138-8. Epub 2018 Jul 23.
  PUBMED    30038252
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0138-8"; journalName: "Nat Cell Biol"; date: 
            "2018-08"; volume: "20"; issue: "8"; pages: "966-978"
COMMENT     Sequence Label: EFIa-iTGB1
FEATURES             Location/Qualifiers
     source          1..11283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    83..200
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    218..238
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        239..1417
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          470..1408
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1365..1385
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1417..1441
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3845..3869)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3887..4475
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3940..3960)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4358..4369)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4541..4564)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4903..4922
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4987..6012
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4987..6012
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6084..6317
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6389..6510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6426..6445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6480..6499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6550..6685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6705..6724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6706..6724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6734..6751)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6734..6750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6743..6765)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6892..7347
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6979..6998)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7189..7210
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7373..7477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7478..7546,7547..8338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7696..7715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8509..9097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8998..9017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9251..9268
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9385..9406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9421..9426,9427..9444,9445..9451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9459..9475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9464..9486
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9483..9499
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9483..9499
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9518..9538
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9520..9538
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9566..9792
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9793..9973
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10020..10145
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10638..10871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11056..11100
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
       61 ggatctcgac ggtatcgcta gcttttaaaa gaaaaggggg gattgggggg tacagtgcag
      121 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      181 ttacaaaaat tcaaaatttt actagtgatt atcggatcaa ctttgtatag aaaagttggg
      241 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      301 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      361 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      421 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      481 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      541 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      601 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      661 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
      721 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
      781 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
      841 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
      901 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
      961 tctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     1021 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     1081 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     1141 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     1201 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1261 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1321 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1381 ggttcaaagt ttttttcttc catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc
     1441 tgccaccatg aatttacaac caattttctg gattggactg atcagttcag tttgctgtgt
     1501 gtttgctcaa acagatgaaa atagatgttt aaaagcaaat gccaaatcat gtggagaatg
     1561 tatacaagca gggccaaatt gtgggtggtg cacaaattca acatttttac aggaaggaat
     1621 gcctacttct gcacgatgtg atgatttaga agccttaaaa aagaagggtt gccctccaga
     1681 tgacatagaa aatcccagag gctccaaaga tataaagaaa aataaaaatg taaccaaccg
     1741 tagcaaagga acagcagaga agctcaagcc agaggatatt actcagatcc aaccacagca
     1801 gttggttttg cgattaagat caggggagcc acagacattt acattaaaat tcaagagagc
     1861 tgaagactat cccattgacc tctactacct tatggacctg tcttactcaa tgaaagacga
     1921 tttggagaat gtaaaaagtc ttggaacaga tctgatgaat gaaatgagga ggattacttc
     1981 ggacttcaga attggatttg gctcatttgt ggaaaagact gtgatgcctt acattagcac
     2041 aacaccagct aagctcagga acccttgcac aagtgaacag aactgcacca gcccatttag
     2101 ctacaaaaat gtgctcagtc ttactaataa aggagaagta tttaatgaac ttgttggaaa
     2161 acagcgcata tctggaaatt tggattctcc agaaggtggt ttcgatgcca tcatgcaagt
     2221 tgcagtttgt ggatcactga ttggctggag gaatgttaca cggctgctgg tgttttccac
     2281 agatgccggg tttcactttg ctggagatgg gaaacttggt ggcattgttt taccaaatga
     2341 tggacaatgt cacctggaaa ataatatgta cacaatgagc cattattatg attatccttc
     2401 tattgctcac cttgtccaga aactgagtga aaataatatt cagacaattt ttgcagttac
     2461 tgaagaattt cagcctgttt acaaggagct gaaaaacttg atccctaagt cagcagtagg
     2521 aacattatct gcaaattcta gcaatgtaat tcagttgatc attgatgcat acaattccct
     2581 ttcctcagaa gtcattttgg aaaacggcaa attgtcagaa ggagtaacaa taagttacaa
     2641 atcttactgc aagaacgggg tgaatggaac aggggaaaat ggaagaaaat gttccaatat
     2701 ttccattgga gatgaggttc aatttgaaat tagcataact tcaaataagt gtccaaaaaa
     2761 ggattctgac agctttaaaa ttaggcctct gggctttacg gaggaagtag aggttattct
     2821 tcagtacatc tgtgaatgtg aatgccaaag cgaaggcatc cctgaaagtc ccaagtgtca
     2881 tgaaggaaat gggacatttg agtgtggcgc gtgcaggtgc aatgaagggc gtgttggtag
     2941 acattgtgaa tgcagcacag atgaagttaa cagtgaagac atggatgctt actgcaggaa
     3001 agaaaacagt tcagaaatct gcagtaacaa tggagagtgc gtctgcggac agtgtgtttg
     3061 taggaagagg gataatacaa atgaaattta ttctggcaaa ttctgcgagt gtgataattt
     3121 caactgtgat agatccaatg gcttaatttg tggaggaaat ggtgtttgca agtgtcgtgt
     3181 gtgtgagtgc aaccccaact acactggcag tgcatgtgac tgttctttgg atactagtac
     3241 ttgtgaagcc agcaacggac agatctgcaa tggccggggc atctgtgagt gtggtgtctg
     3301 taagtgtaca gatccgaagt ttcaagggca aacgtgtgag atgtgtcaga cctgccttgg
     3361 tgtctgtgct gagcataaag aatgtgttca gtgcagagcc ttcaataaag gagaaaagaa
     3421 agacacatgc acacaggaat gttcctattt taacattacc aaggtagaaa gtcgggacaa
     3481 attaccccag ccggtccaac ctgatcctgt gtcccattgt aaggagaagg atgttgacga
     3541 ctgttggttc tattttacgt attcagtgaa tgggaacaac gaggtcatgg ttcatgttgt
     3601 ggagaatcca gagtgtccca ctggtccaga catcattcca attgtagctg gtgtggttgc
     3661 tggaattgtt cttattggcc ttgcattact gctgatatgg aagcttttaa tgataattca
     3721 tgacagaagg gagtttgcta aatttgaaaa ggagaaaatg aatgccaaat gggacacggg
     3781 tgaaaatcct atttataaga gtgccgtaac aactgtggtc aatccgaagt atgagggaaa
     3841 atgaacccag ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga
     3901 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3961 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4021 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4081 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4141 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4201 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4261 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4321 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4381 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4441 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgaatt
     4501 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     4561 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     4621 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     4681 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     4741 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     4801 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     4861 aaggggtggg tccgggggcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     4921 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctcacgtg
     4981 gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     5041 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     5101 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     5161 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     5221 gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     5281 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     5341 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     5401 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     5461 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     5521 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5581 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5641 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     5701 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     5761 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     5821 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     5881 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     5941 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     6001 gcaaaggaat agggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6061 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6121 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6181 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6241 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6301 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6361 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6481 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6541 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6601 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6661 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6721 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6781 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6841 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7321 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7381 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7441 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7501 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7561 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7621 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7681 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7741 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7801 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7861 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7921 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7981 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8041 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8101 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8161 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8221 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8281 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8341 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga gaaaggcgga
     8941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9121 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9181 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9241 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9301 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9361 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9421 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9481 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9541 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     9601 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     9661 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     9721 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     9781 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9961 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10021 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10081 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10141 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10201 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10261 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10321 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10381 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10441 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    10501 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10561 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10621 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10681 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10741 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10801 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10861 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10921 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10981 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    11041 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11101 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11161 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11221 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    11281 ccc
//
