LOCUS       sequence-224021-        8989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral, trimethoprim (TMP) regulated YAP promoter activity 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Er EE, Valiente M, Ganesh K, Zou Y, Agrawal S, Hu J, Griscom B, 
            Rosenblum M, Boire A, Brogi E, Giancotti FG, Schachner M, Malladi S,
            Massague J
  TITLE     Pericyte-like spreading by disseminated cancer cells activates YAP 
            and MRTF for metastatic colonization.
  JOURNAL   Nat Cell Biol. 2018 Aug;20(8):966-978. doi: 
            10.1038/s41556-018-0138-8. Epub 2018 Jul 23.
  PUBMED    30038252
REFERENCE   2  (bases 1 to 8989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0138-8"; journalName: "Nat Cell Biol"; date: 
            "2018-08"; volume: "20"; issue: "8"; pages: "966-978"
COMMENT     Sequence Label: pTRE-8XGTIIC-DsRED-DD
FEATURES             Location/Qualifiers
     source          1..8989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(48..54,55..56,57..63)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(80..86,87..88,89..95)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(134..140,141..142,143..149)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(166..172,173..174,175..181)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     primer_bind     207..223
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     208..224
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      359..368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             365..1039
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(545..565)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     763..782
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     957..980
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             1046..1522
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     protein_bind    complement(1528..1552)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1583..2171
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1636..1656)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2054..2065)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2249..2272)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2601..2620
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2682..3485
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2745..2764)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3355..3374
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             3576..3809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3881..4002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3918..3937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3972..3991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4042..4177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4197..4216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4198..4216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4226..4243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4226..4242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4235..4257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4384..4839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4471..4490)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4681..4702
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4865..4969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4970..5038,5039..5830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5188..5207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6001..6589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6490..6509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6743..6760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6877..6898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6913..6918,6919..6936,6937..6943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6951..6967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6956..6978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6975..6991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6975..6991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7010..7030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7012..7030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7058..7284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7285..7465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7512..7637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8130..8363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8548..8592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8859..8976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taccgagctc ttacgcgtgc tagcccggcc agtgccaagt tgagacacat tccacacatt
       61 ccactgcaag cttgagacac attccacaca ttccactgca agcttctagc ccggccagtg
      121 ccaagttgag acacattcca cacattccac tgcaagcttg agacacattc cacacattcc
      181 actgcaagct tctagagatc tgcaggtcga ggtcgacggt atcgataagc ttgggggtgg
      241 gcgccggggg gaccttaaag cctctgcccc ccaaggagcc cttcccagac agccgccggc
      301 acccaccgct ccgtgggacg atcccccaag agcttggcat tccggtactg ttggtaaagc
      361 caccatggat agcactgaga acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga
      421 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggca agccctacga
      481 gggcacccag accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga
      541 catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat
      601 ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      661 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcaccttcat
      721 ctaccacgtg aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa
      781 gactctgggc tgggagccct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
      841 cgagatccac aaggcgctga agctgaaggg cggcggccac tacctggtgg agttcaagtc
      901 aatctacatg gccaagaagc ccgtgaagct gcccggctac tactacgtgg actccaagct
      961 ggacatcacc tcccacaacg aggactacac cgtggtggag cagtacgagc gcgccgaggc
     1021 ccgccaccac ctgttccagc tcgagatcag tctgattgcg gcgttagcgg tagatcacgt
     1081 tatcggcatg gaaaccgtca tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg
     1141 caacacctta aataaacccg tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc
     1201 gttgccagga cgcaaaaata ttatcctcag cagtcaaccg agtacggacg atcgcgtaac
     1261 gtgggtgaag tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt
     1321 tattggcggc ggtcgcgttt atgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac
     1381 gcatatcgac gcagaagtgg aaggcgacac ccatttcccg gattacgagc cggatgactg
     1441 ggaatcggta ttcagcgaat tccacgatgc tgatgcgcag aactctcaca gctattgctt
     1501 tgagattctg gagcggcgat gaactagacc cagctttctt gtacaaagtg gttgatatcc
     1561 agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     1621 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1681 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1741 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1801 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1861 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     1921 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     1981 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2041 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2101 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2161 cgcctccccg cctggaattc tgcagatatc cggactagtg atctaattct accgggtagg
     2221 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     2281 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     2341 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     2401 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     2461 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     2521 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     2581 cgggggctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     2641 ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa tatgggatcg gccattgaac
     2701 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     2761 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     2821 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     2881 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     2941 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3001 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3061 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3121 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     3181 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     3241 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     3301 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     3361 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     3421 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3481 tctgagggga tccgtcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt
     3541 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     3601 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3661 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3721 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3781 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     3841 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     3901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     4021 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4081 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4141 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     4201 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4261 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4321 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4381 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4441 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4501 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4561 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4621 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4681 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4741 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4801 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     4861 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4921 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4981 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5041 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5101 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5161 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5221 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5281 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5341 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5401 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5461 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5521 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5581 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5641 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5701 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5761 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5821 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5881 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5941 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6001 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6061 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     6841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     6901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     6961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     7261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     7321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     7501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     7561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     7621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     7681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     7741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     7801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     7861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     7921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     7981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     8461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     8521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     8581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     8641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     8701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     8761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     8821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     8881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     8941 gaattacaaa aacaaattac aaaaattcaa aattttatcg aacgatagg
//
