LOCUS       sequence-224026-       10261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LRP5 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky 
            MW, Chen Z, Junge HJ
  TITLE     TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand 
            Selectivity and Signaling.
  JOURNAL   Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 
            10.1016/j.celrep.2017.06.004.
  PUBMED    28658627
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-06-27- 27"; volume: "19"; issue: "13"; pages: "2809-2822"
COMMENT     Sequence Label: pcDNA 3.3 LRP5
FEATURES             Location/Qualifiers
     source          1..10261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5647..5666)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5691..5739
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5941..6369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6028..6047)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6238..6259
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6378..6398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6383..6712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6563..6698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6625..6644
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6779..7573
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6833..6852)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7443..7462
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7749..7870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7786..7805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7840..7859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7919..7935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7919..7935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7932..7954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7943..7959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7967..7973,7974..7991,7992..7997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8012..8033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8150..8167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8321..8909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8401..8420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9080..9871,9872..9940))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9703..9722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9941..10045)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10120..10139)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagg atcgaaccct tccaccatgg aggcagcgcc gcccgggccg ccgtggccgc
      781 tgctgctgct gctgctgctg ctgctggcgc tgtgcggctg cccggccccc gccgcggcct
      841 cgccgctcct gctatttgcc aaccgccggg acgtacggct ggtggacgcc ggcggagtca
      901 agctggagtc caccatcgtg gtcagcggcc tggaggatgc ggccgcagtg gacttccagt
      961 tttccaaggg agccgtgtac tggacagacg tgagcgagga ggccatcaag cagacctacc
     1021 tgaaccagac gggggccgcc gtgcagaacg tggtcatctc cggcctggtc tctcccgacg
     1081 gcctcgcctg cgactgggtg ggcaagaagc tgtactggac ggactcagag accaaccgca
     1141 tcgaggtggc caacctcaat ggcacatccc ggaaggtgct cttctggcag gaccttgacc
     1201 agccgagggc catcgccttg gaccccgctc acgggtacat gtactggaca gactggggtg
     1261 agacgccccg gattgagcgg gcagggatgg atggcagcac ccggaagatc attgtggact
     1321 cggacattta ctggcccaat ggactgacca tcgacctgga ggagcagaag ctctactggg
     1381 ctgacgccaa gctcagcttc atccaccgtg ccaacctgga cggctcgttc cggcagaagg
     1441 tggtggaggg cagcctgacg caccccttcg ccctgacgct ctccggggac actctgtact
     1501 ggacagactg gcagacccgc tccatccatg cctgcaacaa gcgcactggg gggaagagga
     1561 aggagatcct gagtgccctc tactcaccca tggacatcca ggtgctgagc caggagcggc
     1621 agcctttctt ccacactcgc tgtgaggagg acaatggcgg ctgctcccac ctgtgcctgc
     1681 tgtccccaag cgagcctttc tacacatgcg cctgccccac gggtgtgcag ctgcaggaca
     1741 acggcaggac gtgtaaggca ggagccgagg aggtgctgct gctggcccgg cggacggacc
     1801 tacggaggat ctcgctggac acgccggact tcaccgacat cgtgctgcag gtggacgaca
     1861 tccggcacgc cattgccatc gactacgacc cgctagaggg ctatgtctac tggacagatg
     1921 acgaggtgcg ggccatccgc agggcgtacc tggacgggtc tggggcgcag acgctggtca
     1981 acaccgagat caacgacccc gatggcatcg cggtcgactg ggtggcccga aacctctact
     2041 ggaccgacac gggcacggac cgcatcgagg tgacgcgcct caacggcacc tcccgcaaga
     2101 tcctggtgtc ggaggacctg gacgagcccc gagccatcgc actgcacccc gtgatgggcc
     2161 tcatgtactg gacagactgg ggagagaacc ctaaaatcga gtgtgccaac ttggatgggc
     2221 aggagcggcg tgtgctggtc aatgcctccc tcgggtggcc caacggcctg gccctggacc
     2281 tgcaggaggg gaagctctac tggggagacg ccaagacaga caagatcgag gtgatcaatg
     2341 ttgatgggac gaagaggcgg accctcctgg aggacaagct cccgcacatt ttcgggttca
     2401 cgctgctggg ggacttcatc tactggactg actggcagcg ccgcagcatc gagcgggtgc
     2461 acaaggtcaa ggccagccgg gacgtcatca ttgaccagct gcccgacctg atggggctca
     2521 aagctgtgaa tgtggccaag gtcgtcggaa ccaacccgtg tgcggacagg aacggggggt
     2581 gcagccacct gtgcttcttc acaccccacg caacccggtg tggctgcccc atcggcctgg
     2641 agctgctgag tgacatgaag acctgcatcg tgcctgaggc cttcttggtc ttcaccagca
     2701 gagccgccat ccacaggatc tccctcgaga ccaataacaa cgacgtggcc atcccgctca
     2761 cgggcgtcaa ggaggcctca gccctggact ttgatgtgtc caacaaccac atctactgga
     2821 cagacgtcag cctgaagacc atcagccgcg ccttcatgaa cgggagctcg gtggagcacg
     2881 tggtggagtt tggccttgac taccccgagg gcatggccgt tgactggatg ggcaagaacc
     2941 tctactgggc cgacactggg accaacagaa tcgaagtggc gcggctggac gggcagttcc
     3001 ggcaagtcct cgtgtggagg gacttggaca acccgaggtc gctggccctg gatcccacca
     3061 agggctacat ctactggacc gagtggggcg gcaagccgag gatcgtgcgg gccttcatgg
     3121 acgggaccaa ctgcatgacg ctggtggaca aggtgggccg ggccaacgac ctcaccattg
     3181 actacgctga ccagcgcctc tactggaccg acctggacac caacatgatc gagtcgtcca
     3241 acatgctggg tcaggagcgg gtcgtgattg ccgacgatct cccgcacccg ttcggtctga
     3301 cgcagtacag cgattatatc tactggacag actggaatct gcacagcatt gagcgggccg
     3361 acaagactag cggccggaac cgcaccctca tccagggcca cctggacttc gtgatggaca
     3421 tcctggtgtt ccactcctcc cgccaggatg gcctcaatga ctgtatgcac aacaacgggc
     3481 agtgtgggca gctgtgcctt gccatccccg gcggccaccg ctgcggctgc gcctcacact
     3541 acaccctgga ccccagcagc cgcaactgca gcccgcccac caccttcttg ctgttcagcc
     3601 agaaatctgc catcagtcgg atgatcccgg acgaccagca cagcccggat ctcatcctgc
     3661 ccctgcatgg actgaggaac gtcaaagcca tcgactatga cccactggac aagttcatct
     3721 actgggtgga tgggcgccag aacatcaagc gagccaagga cgacgggacc cagccctttg
     3781 ttttgacctc tctgagccaa ggccaaaacc cagacaggca gccccacgac ctcagcatcg
     3841 acatctacag ccggacactg ttctggacgt gcgaggccac caataccatc aacgtccaca
     3901 ggctgagcgg ggaagccatg ggggtggtgc tgcgtgggga ccgcgacaag cccagggcca
     3961 tcgtcgtcaa cgcggagcga gggtacctgt acttcaccaa catgcaggac cgggcagcca
     4021 agatcgaacg cgcagccctg gacggcaccg agcgcgaggt cctcttcacc accggcctca
     4081 tccgccctgt ggccctggtg gtagacaaca cactgggcaa gctgttctgg gtggacgcgg
     4141 acctgaagcg cattgagagc tgtgacctgt caggggccaa ccgcctgacc ctggaggacg
     4201 ccaacatcgt gcagcctctg ggcctgacca tccttggcaa gcatctctac tggatcgacc
     4261 gccagcagca gatgatcgag cgtgtggaga agaccaccgg ggacaagcgg actcgcatcc
     4321 agggccgtgt cgcccacctc actggcatcc atgcagtgga ggaagtcagc ctggaggagt
     4381 tctcagccca cccatgtgcc cgtgacaatg gtggctgctc ccacatctgt attgccaagg
     4441 gtgatgggac accacggtgc tcatgcccag tccacctcgt gctcctgcag aacctgctga
     4501 cctgtggaga gccgcccacc tgctccccgg accagtttgc atgtgccaca ggggagatcg
     4561 actgtatccc cggggcctgg cgctgtgacg gctttcccga gtgcgatgac cagagcgacg
     4621 aggagggctg ccccgtgtgc tccgccgccc agttcccctg cgcgcggggt cagtgtgtgg
     4681 acctgcgcct gcgctgcgac ggcgaggcag actgtcagga ccgctcagac gaggcggact
     4741 gtgacgccat ctgcctgccc aaccagttcc ggtgtgcgag cggccagtgt gtcctcatca
     4801 aacagcagtg cgactccttc cccgactgta tcgacggctc cgacgagctc atgtgtgaaa
     4861 tcaccaagcc gccctcagac gacagcccgg cccacagcag tgccatcggg cccgtcattg
     4921 gcatcatcct ctctctcttc gtcatgggtg gtgtctattt tgtgtgccag cgcgtggtgt
     4981 gccagcgcta tgcgggggcc aacgggccct tcccgcacga gtatgtcagc gggaccccgc
     5041 acgtgcccct caatttcata gccccgggcg gttcccagca tggccccttc acaggcatcg
     5101 catgcggaaa gtccatgatg agctccgtga gcctgatggg gggccggggc ggggtgcccc
     5161 tctacgaccg gaaccacgtc acaggggcct cgtccagcag ctcgtccagc acgaaggcca
     5221 cgctgtaccc gccgatcctg aacccgccgc cctccccggc cacggacccc tccctgtaca
     5281 acatggacat gttctactct tcaaacattc cggccactgc gagaccgtac aggccctaca
     5341 tcattcgagg aatggcgccc ccgacgacgc cctgcagcac cgacgtgtgt gacagcgact
     5401 acagcgccag ccgctggaag gccagcaagt actacctgga tttgaactcg gactcagacc
     5461 cctatccacc cccacccacg ccccacagcc agtacctgtc ggcggaggac agctgcccgc
     5521 cctcgcccgc caccgagagg agctacttcc atctcttccc gccccctccg tccccctgca
     5581 cggactcatc ctgacaaggg ttcgatccct accggttagt aatgagttta aacgggggag
     5641 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     5701 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     5761 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     5821 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     5881 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc
     5941 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6001 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6061 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6121 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6181 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6241 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6301 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6361 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6541 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6661 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6721 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     6781 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6841 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6901 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6961 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7021 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7081 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7141 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7201 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7261 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7321 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7381 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7441 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7501 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7561 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     7621 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7681 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7741 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7801 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7861 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7921 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7981 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8041 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8101 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8761 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10081 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
    10141 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
    10201 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    10261 g
//
