LOCUS       sequence-224038-        3263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab7a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab7a
FEATURES             Location/Qualifiers
     source          1..3263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(649..744)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(781..800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(782..800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(805..821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(805..821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(923..942)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             934..1743
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1001..1020)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1890..2478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2379..2398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2632..2649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2808..2835)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2927..3013)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3062..3084
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3076..3093
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3077..3093
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgacat caaggaagaa agttcttctg aaggtgatca
       61 ttcttggaga ttccggggtt ggaaaaacat ctctaatgaa ccagtatgtg aataaaaagt
      121 tcagtaatca gtataaagcc actataggag cagatttcct gacaaaagaa gtgatggttg
      181 acgaccggct tgtcacaatg cagatatggg acacagcagg tcaggagcgg tttcagtctc
      241 tgggtgttgc tttctatcgt ggtgctgact gctgcgtcct ggtgtttgac gtcactgccc
      301 cgaacacctt caagacactg gacagctgga gggatgagtt tctgatccag gccagtcctc
      361 gagacccaga aaacttccct ttcgtggtgc tgggcaacaa aatcgatctg gagaatagac
      421 aggtaacaac taagcgagca caagcttggt gtcagagtaa gaacaacatc ccatattttg
      481 agaccagtgc aaaggaggcc atcaacgtag agcaggcatt ccagaccatc gcgcgcaatg
      541 cactcaaaca ggaaactgaa gtggagttat acaacgagtt ccccgagcca attaaactag
      601 accggaacga tagagccaaa ccatcagcag agacctgtag ctgctgacca actttattat
      661 acaaagttgg cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta
      721 tcagtcaaaa taaaatcatt atttggagct ccatggtagc gttaacgcgg ccgcgatatc
      781 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
      841 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
      901 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
      961 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1021 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1081 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1141 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1201 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1261 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1321 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1381 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1441 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     1501 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1561 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1621 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1681 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     1741 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     1801 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     1861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2641 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2701 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     2761 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     2821 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     2881 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     2941 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3001 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3061 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccctg cagctctaga
     3121 gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg actgcatatg
     3181 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3241 atttatatca ttttacgttt ctc
//
