LOCUS       sequence-224040-        3253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab7b
FEATURES             Location/Qualifiers
     source          1..3253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(739..834)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(871..890)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(872..890)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(895..911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(895..911)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1013..1032)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1024..1833
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1091..1110)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1980..2568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2469..2488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2722..2739
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2898..2925)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3017..3103)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3152..3174
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3166..3183
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3167..3183
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggctt cccgtaaaaa ggtcctcctc aaagtgatca tcctagggga ttctggagtt
      181 gggaagacct ctttgatgaa ccagtatgtg aataataagt tcagtaatca gtataaggcc
      241 accattggtg cagactttct gacaaaggag gtgatggtgg acgacagact ggtgaccatg
      301 cagatttggg acacagccgg tcaggagcgg tttcagtctc tgggcgtggc cttctacaga
      361 ggggcggact gttgtgtgct ggtgtatgat gtcacggcgc ccaccacgtt taagactctg
      421 gacagctgga gggacgagtt tctcattcag gccagtacaa gcgacccgga gaacttcccc
      481 tttgttgtgc tgggcaacaa aatcgacctg gacaaccgac aggtatcgac aaagcgagca
      541 caagcgtggt gccagagtaa gaacaacatt ccctattttg agaccagcgc caaagaagcc
      601 attaatgtgg aacaggcctt ccagaccatc gctcgcaatg cgcttaaaca ggagtcggtg
      661 gacaaatatg atttcccgga cgagatcaaa ctaggaaacg acagacccat gtctaacggt
      721 gaaggctgca gctgctgaca actttattat acaaagttgg cattataaaa aagcattgct
      781 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggagct
      841 ccatggtagc gttaacgcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata
      901 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
      961 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1021 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1081 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1141 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1201 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1261 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1321 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1381 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1441 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1501 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1561 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1621 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     1681 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     1741 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     1801 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     1861 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     1921 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1981 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2041 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2101 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2161 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2221 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2281 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2341 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2401 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2461 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2521 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2581 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2641 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2701 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2761 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2821 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     2881 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     2941 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3001 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     3061 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     3121 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     3181 agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac taataccatc
     3241 taagtagttg gtt
//
