LOCUS       sequence-224045-        3274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab8b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab8b
FEATURES             Location/Qualifiers
     source          1..3274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(660..755)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(792..811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(816..832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(816..832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(934..953)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             945..1754
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1012..1031)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1901..2489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2390..2409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2643..2660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2819..2846)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2938..3024)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3073..3095
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3087..3104
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3088..3104
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggcga aaacgtacga ctatctgttc aaactgctgc
       61 tcatcggaga cagcggtgtg ggcaagacct gtctgctgtt tcgattcagc gaggacgcct
      121 tcaacaccac tttcatctcc accataggaa ttgacttcaa aatcagaaca attgagctgg
      181 atgggaaaaa gatcaagctt caaatctggg atacggcggg acaggagagg ttcagaacca
      241 tcaccactgc atattacaga ggagcaatgg gaattatgct ggtgtatgat atcacaaatg
      301 agaaatcatt tgacaacatc aagaactgga tcaggaacat tgaggagcat gcatcgtcag
      361 atgtggagcg aatgatattg ggtaacaggt gtgacatgaa cgacaagaga caagtttcaa
      421 aagaaagagg agagaagcta gctattgatt acggaatcaa gttcttggaa accagtgcaa
      481 aatctagcac aaatgttgaa gaggcatttg tcactcttgc tagagacatc atgacgagat
      541 taaacaggaa aatgaatgag aataaccctt cagggggagg aggaggagga gcagtaaaaa
      601 tcacagagag tcgatccaag aagccaagct tcttccgctg cattctgctc taacccagcc
      661 aactttatta tacaaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga
      721 acaggtcact atcagtcaaa ataaaatcat tatttggagc tccatggtag cgttaacgcg
      781 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
      841 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
      901 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
      961 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1021 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     1081 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1141 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     1201 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     1261 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     1321 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     1381 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     1441 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     1501 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     1561 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     1621 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     1681 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     1741 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     1801 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     1861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2041 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2281 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2641 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2701 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2761 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     2821 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     2881 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     2941 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     3001 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     3061 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccct
     3121 gcagctctag agctcgaatt ctacaggtca ctaataccat ctaagtagtt ggttcatagt
     3181 gactgcatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     3241 aatatattga tatttatatc attttacgtt tctc
//
