LOCUS       sequence-224046-        3244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab9a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab9a
FEATURES             Location/Qualifiers
     source          1..3244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(630..725)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(762..781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(763..781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(904..923)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             915..1724
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(982..1001)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1871..2459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2360..2379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2613..2630
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2789..2816)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2908..2994)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3043..3065
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3057..3074
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3058..3074
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtgtcat ctaaatcatc tctcctgaaa gtgattctgc
       61 tgggtgatgg cggcgtgggc aaaagctcgc tcatgaaccg atatgtcacc aacaagtttg
      121 atgctcatct cttccacacc atcggtgtgg agttcctcaa taaagacttg gaggtggacg
      181 gccgtactgt gaccctgcag atctgggata cagccggtca ggagcgcttc cgtagcctcc
      241 gcacgccttt ctaccgcggc tccgactgct gcctgctgac cttcagtgtg gatgacagcc
      301 agagcttcca caacctggtc aactggaaaa aagagttcat ttattacgcc gacgtcaagg
      361 agcccgaaag cttcccgttt gtggttctgg gaaataaact ggacgtttcg gagcgtcagg
      421 tgagcagtga agaagcgcag gagtggtgca tggagagcgg tggataccca tatttcgaga
      481 ccagtgcaaa agatgccact aatgtcgcgg ttgcgttcga ggaggcggtg agaagagtgc
      541 tgtctctgga ggacagacac gagcatctga ttcctacaga cactgtgaat ctgcacagga
      601 aaccgcgcag cgctacacag tgctgttaac aactttatta tacaaagttg gcattataaa
      661 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      721 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt
      781 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      841 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      901 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
      961 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1021 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1081 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1141 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1201 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1261 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1321 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     1381 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1441 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1501 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1561 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1621 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1681 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1741 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     1801 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     1861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1981 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2521 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2581 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2641 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2701 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     2761 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     2821 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     2881 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     2941 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     3001 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     3061 aaacgacggc cagtcttaag ctcgggccct gcagctctag agctcgaatt ctacaggtca
     3121 ctaataccat ctaagtagtt ggttcatagt gactgcatat gttgtgtttt acagtattat
     3181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     3241 tctc
//
