LOCUS       sequence-224052-        8641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miR-292 regulated GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Sachdeva R, Jonsson ME, Nelander J, Kirkeby A, Guibentif C, Gentner 
            B, Naldini L, Bjorklund A, Parmar M, Jakobsson J
  TITLE     Tracking differentiating neural progenitors in pluripotent cultures 
            using microRNA-regulated lentiviral vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11602-7. doi: 
            10.1073/pnas.1006568107. Epub 2010 Jun 7.
  PUBMED    20534548
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1006568107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-06-22- 22"; volume: "107"; issue: "25"; pages: "11602-7"
COMMENT     Sequence Label: pLV.UBI.GFP.miR292T
FEATURES             Location/Qualifiers
     source          1..8641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..90
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(243..248,249..266,267..273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     286..308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     305..321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     305..321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     340..360
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        342..360
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        461..840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        841..1044
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     995..1015
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1059..1239
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1286..1411
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1904..2137
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2322..2366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2664..2781
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2846..4057
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3894..3913
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4105..4114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4111..4113,4114..4116,4117..4830)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4111..4113,4114..4116,4117..4827)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4156..4177)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4417..4436)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4764..4785
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4899..4919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5317..5328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5568..5801
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5873..5994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5910..5929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5964..5983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6013..6148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6168..6187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6169..6187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6197..6214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6197..6213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6206..6228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6355..6810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6442..6461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6652..6673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6836..6940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6941..7009,7010..7801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7159..7178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7972..8560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8461..8480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
       61 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      301 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      361 gaacaaaagc tggagctgca agcttggcca ttgcatacgt tgtatccata tcataatatg
      421 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      481 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      541 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      601 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      661 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      721 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      781 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      841 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      901 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      961 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1021 tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg ttagaccaga
     1081 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1141 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1201 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1261 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1321 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1381 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1441 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1501 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1561 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1621 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1681 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1741 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1801 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1861 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1921 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1981 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     2041 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2101 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2161 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2221 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2281 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2341 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2401 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2461 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2521 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2581 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     2641 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2701 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2761 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     2821 attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     2881 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     2941 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     3001 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     3061 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     3121 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     3181 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     3241 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     3301 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     3361 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     3421 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     3481 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     3541 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     3601 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     3661 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     3721 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     3781 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     3841 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     3901 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     3961 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     4021 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgact
     4081 ctagaggatc cccgggtacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     4141 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4201 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4261 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4321 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4381 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4441 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4501 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4561 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4621 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4681 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4741 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4801 actctcggca tggacgagct gtacaagtaa agcggccgcg tcgacaatca acctctggat
     4861 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4921 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4981 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5041 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5101 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5161 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5221 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5281 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5341 ccttcccgcg gcctgctgcc ggctctagat aaacactcaa aacctggcgg cacttttcga
     5401 aacactcaaa acctggcggc acttacgcgt acactcaaaa cctggcggca cttatgcata
     5461 cactcaaaac ctggcggcac ttcccgggtc gagctcggta cctttaagac caatgactta
     5521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5581 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5821 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5881 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5941 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6001 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6061 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6121 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     6181 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6301 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6361 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6421 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6481 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6541 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6601 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6661 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6721 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6781 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6901 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6961 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7021 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7081 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7141 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7201 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7261 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7321 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7381 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7441 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7501 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7561 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7621 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7681 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7741 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8641 t
//
