LOCUS       sequence-22406-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Jen Y, Manova K, Benezra R
  TITLE     Expression patterns of Id1, Id2, and Id3 are highly related but 
            distinct from that of Id4 during mouse embryogenesis.
  JOURNAL   Dev Dyn. 1996 Nov . 207(3):235-52.
  PUBMED    8922523
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            1996 Nov . 207(3):235-52."
COMMENT     Sequence Label: pBS/KS-mId2sp
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(235..251)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(236..252)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(280..298)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(319..335)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(319..335)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(332..354)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    343..359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(367..373,374..391,392..397))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    412..433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(550..567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggccgctct agactagtgg atccatgctg ctcgtaggag gagagaccgg gagggagccg
       61 ccacaggggg ctgagctgac tgcgcggcac caggctcggt tcagaatgaa gcgcgagccg
      121 ggcggggcgt cttttatgtg cactcgccct ggccgctcaa tcgggcatcc ctgaattgcc
      181 attggtggaa gggctcccgt ccatcatacc aggcgaattc gatatcaagc ttatcgatac
      241 cgtcgacctc gagggggggc ccggtaccag cttttgttcc ctttagtgag ggttaatttc
      301 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      361 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      421 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      481 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg cangaaagaa
      661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aggccgcgtt gctggcgttt
      721 ttcataggct ccgcccccct gacgagcatc acaaaatcga cgc
//
