LOCUS       sequence-22407-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Jen Y, Manova K, Benezra R
  TITLE     Expression patterns of Id1, Id2, and Id3 are highly related but 
            distinct from that of Id4 during mouse embryogenesis.
  JOURNAL   Dev Dyn. 1996 Nov . 207(3):235-52.
  PUBMED    8922523
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            1996 Nov . 207(3):235-52."
COMMENT     Sequence Label: pBS/KS-mId3sp
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(264..280)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(265..281)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(309..327)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(348..364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(348..364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(361..383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    372..388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(396..402,403..420,421..426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    441..462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(579..596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaggagcttt tgccactgac ccggtcgtcc tggcacctcc cgaacgcagg tgctggcgcc
       61 cgttccgctt gggaccctgg gactctggga ctctgggacc ctctctccag ccggaagcct
      121 gagggcatgg atgagcttcg atcttaaccc agccctcttc acttaccctg aactcaacgc
      181 ctcgaggctg gacctggagc ccgagagaag gactgaactt gggtggcctg aagagctagc
      241 acagaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtaccagc
      301 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
      361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      421 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      541 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      601 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      661 acagaatc
//
