LOCUS       sequence-224079-       20530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20530)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 20530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU74.2-2.2
FEATURES             Location/Qualifiers
     source          1..20530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(911..922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      1802..2101
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4713..4933
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4934..5737
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      12347..12534
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(12402..12426)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      14141..14390
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(14157..14175)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(16378..16398)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16378..16396)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(16735..17323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16815..16834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17494..18285,18286..18354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18117..18136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18355..18459)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      18486..19828
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(19449..19496)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     19869..19887
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(20229..20245)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(20229..20245)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aggcagagga gagcatagaa atggggttca ctttttggta aagctatagc atgcctatca
       61 catataaata gagtgccagt agcgactttt ttcacactcg aaatactctt actactgctc
      121 tcttgttgtt tttatcactt cttgtttctt cttggtaaat agaatatcaa gctacaaaaa
      181 gcatacaatc aactatcaac tattaactat atcgtaatac acaatggcga cagtgcatct
      241 tcacgatgag ttgacgcaat ctacggaacg ggtcacggtc cagacgcgct ttggcacgat
      301 cagaggcggg cgtgctgcga atggcgccgc ggtcttcttg gaggttccgt acgctctccc
      361 cccggggagg tttttggacc cggtcccatt gcccgcggac tatcagtatg aggagaagga
      421 ttacatctac gaaagcagct acgcggctca accgattaat gacggtcagg cagctagtgt
      481 gccattcgag gacaaagtcg gacgaggcga acccactgag aaccctctgt tcgtcaacat
      541 agtatccccg ccgtcgtttc cctctgcgac tggcttgcct gtgaaagtgt acatccacgg
      601 cggcttcttg cagtttgggt cgcctcacgg tctaagtggg caagcgcaat acattgcggc
      661 cgagcgatcg gaaatatggg tcaacgtcgg ataccgcctt tccgcgtttg gcttcctggc
      721 atgcgacgag ccacgcgtcg aaggaaactt tggtttcaag gaccagtggt tggcgctgct
      781 atgggtccgt gacaacatcg aggcattcgg aggcaatcca gacaacatcc aaatcagtgg
      841 tctttccgcg ggtgcccatt ctgtacatca gcttctgcat tacgcttcga ggctgccaga
      901 gggcaaaacg gcgcccttca attctgcagt tctgcagtcg aactccattg taatagcccc
      961 gaaaaccccc gccgagctgc gcccgcaatt ccgcgcgttc tgcaccgcgc tctccctcaa
     1021 cccggacggc cccgacgcct taaccgtcct gcgcgacccg gagctggtcc cctggaaggc
     1081 catcacgcac gcgatcgaaa ccgacgcgct cgggacccag tttggcacct tccgcggcag
     1141 cctcgacggc tcctggcttt cctctgatcc ggaatcaatg gcctggcagc gctccggcgc
     1201 gttcgcccgc gcgttgcgaa agcgtggtgt gcggagcatc gcggtgggcg acctcacgga
     1261 ggaatggtac ctgtactcga tcgcgcaccc cgtgcggcgc atgcgtgata ttgagctgaa
     1321 catggagcgg tattaccagg aggaggtcgt ccacgccatg atggcgctgt acaggcgcgt
     1381 ccccgacggc gcgcctcccg aggattttgc gcgcctgttt ggggagatgc tgagcgaagg
     1441 ccaggtgcat ttgcctgtgc ggttgctcgc gagggacctc acgacggctg ggttcccggt
     1501 ggtgcggtac cagattcgat ggacgcccga gcagctgagg cctctcggat atgtcacgca
     1561 tggaagcgac cggtcgctct gggcgcttcg tctacccgtg ctcaagccag accaggccga
     1621 tgttgcgcgt gcgtggctgg acagcatcaa ttccgcactc gctgcggcgg agaacgggga
     1681 ggtacgaggg ctgaaggagg ttctggcttt gaatgaaaac caaagtatcg gatggagaga
     1741 cgatgacaag tgggacgaga tcatgcgcct ttgtcaggcg ttgccggggg aggatgcata
     1801 agcggatctc ttatgtcttt acgatttata gttttcatta tcaagtatgc ctatattagt
     1861 atatagcatc tttagatgac agtgttcgaa gtttcacgaa taaaagataa tattctactt
     1921 tttgctccca ccgcgtttgc tagcacgagt gaacaccatc cctcgcctgt gagttgtacc
     1981 cattcctcta aactgtagac atggtagctt cagcagtgtt cgttatgtac ggcatcctcc
     2041 aacaaacagt cggttatagt ttgtcctgct cctctgaatc gtctccctcg atatttctca
     2101 ttttccttcg catgccagca ttgaaatgat cgaagttcaa tgatgaaacg gtaattcttc
     2161 tgtcatttac tcatctcatc tcatcaagtt atataattct atacggatgt aatttttcac
     2221 ttttcgtctt gacgtccacc ctataatttc aattattgaa ccctcacgat ccagttctcc
     2281 agtgacacag cctttatctg gtcaaacctt tctttctaat cacctatgct gatgcttaat
     2341 taagggattt ttgtctccat caacggcatg cgcccaaaaa tgacgttttt tttaacccat
     2401 agacacgaaa ctacccattt tccaccggcc tgacctacca ccggaacaac ggccatctcc
     2461 aacttgcaag ttggggaaat taagagcatc gcaggtttaa tggaagaaaa aaaaaaggta
     2521 cagcacagcg caaatggagt tagttccctt atgtcacaca ctcacacaca gtcggtcaga
     2581 tcaagcatac tgggtgcgta taaatagagt ggccattgcc accctgttta tctcaaaatc
     2641 tgtcttgtta gtggtcttct ccctttttca ggttacaatt ctcttgtttc tacttagtat
     2701 ataagtatat caagctatat taagcatact atcaactgtc aactctatcc tcaaaataca
     2761 atacaaaatg ttcaacttac tggactccgc caaaggctcg cacgcaacga cgttcctcgt
     2821 cgcctcactt gttttcctcg tcttgaagca gttcttcacc tcgcgaaaga tggccaagcg
     2881 agcaccgggt ccgacgcgct ggccattcct cggcaatgct ctccaacttc ctcaacaaca
     2941 gatgtggctg accttctcgg aatggcaaaa gacatacggc gacattattc acgtcgacgt
     3001 cttcaaccag ccgatcatca ttataaactc ctcgaagata gcgaaggatc tcttggacaa
     3061 gcggtcctcc atctattccg accgcccgca tctggtcatg gccggtgatt tagtgggcta
     3121 cagcgacccg tttgtcctgc aaccttacgg agaaaagtgg aaaaagcagc gcaaatttgt
     3181 cgcatcagac ttcagtcccg ccaccatgcc acggtattac cagctccaag agaccgaggt
     3241 tcgcaaactg gtccgcaatg tcatcttgga cccagattcc ctcttctcgc agactaaatt
     3301 gcggattggg acaattatca tccgggttac ctacgggtac tacatcaagg gcgagcagga
     3361 cccgttcctg actttgcctt tgactgcgat ggaaaacttc agcaaggcat cggctccagg
     3421 gacatggctc gttgacttcg tgccgcagat gaagaacatg ccctcctgga tgcccggcgc
     3481 gggctttctc aagacggcca agaaatggcg caagatcgtc tgggatgcgt cctgggaccc
     3541 atacaactgg tgcaagaatg gccttgaaac cggagagacg ctcatgccaa acctctgcgg
     3601 gagcgctatc cagtccgccg cagatggaaa gctctccaag gaggacgagg agcagctcgt
     3661 atgggccgcg tccgcaatca tgggcggcgg cctcgacacg aacatgtccg cggcgttgag
     3721 cttcttcctc gcgatggtgc tgcatcccgc cgtgcagagg aaagcgcagg ccgagatcga
     3781 cgcggtgatc ggctgcgaac ggctcccgtc catcgcggac cgcgacgcgt tgccatacgt
     3841 gcgcagcatc atcacggagg tcctgcgctg gaagccgccc gtcccgctca gtatcccgca
     3901 ctcgctcacg caggacgacg tgtacgaggg catcttcctg cccaagggcg ccctcatcat
     3961 gcccaacgtt tggcacatga tgcacgaccc cacgtcctac ccagatccct ccgccttccg
     4021 ccccgagcgc tacgcgggtg cggacggtga cgcagcgcag cgccgcgtgc acgagcttgt
     4081 gttcggcttc ggccggcgtg cgtgccccgg cgtgcacttc gccgagggca cgctcttcgc
     4141 gcttgtcgcg accgtcctcg cgactgtcga cgtccttcct gcgctcgacg cgcagggcag
     4201 ggaaatcagg cccgacccga agtactcgtc tggtacgatc acgttcccgg aggcgttccc
     4261 ggtgcggttc agtgtgcgtt cggagcaggc ggaaacgctc ctagcgcagg ttccggcggg
     4321 tcctatgtga acaaatcgct cttaaatata tacctaaaga acattaaagc tatattataa
     4381 gcaaagatac gtaaattttg cttatattat tatacacata tcatatttct atatttttaa
     4441 gatttggtta tataatgtac gtaatgcaaa ggaaataaat tttatacatt attgaacagc
     4501 gtccaagtaa ctacattatg tgcactaata gtttagcgtc gtgaagactt tattgtgtcg
     4561 cgaaaagtaa aaattttaaa aattagagca ccttgaactt gcgaaaaagg ttctcatcaa
     4621 ctgtttaaaa ggaggatatc aggtcctatt tctgacaaac aatatacaaa tttagtttca
     4681 aagatgaatc agtgcgcgaa ggacataact cattcaattc aattcatcat ttttttttta
     4741 ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa
     4801 ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga
     4861 agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa
     4921 acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc
     4981 tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt
     5041 ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg
     5101 tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc
     5161 gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga
     5221 cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc
     5281 agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc
     5341 ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa
     5401 gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga agagcgacaa
     5461 agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga
     5521 ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca
     5581 gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg
     5641 actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg
     5701 ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat aagtaaatgc
     5761 atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat taccctatgc
     5821 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggata ggaaaaaacc
     5881 gagcttcctt tcatccggcg cggctgtgtt ctacatatca ctgaagctcc gggtatttta
     5941 agttatacaa gggaaagatg ccggctagac tagcaagttt taggctgctt aacattatgg
     6001 ataggcggat aaagggccca aacaggattg taaagcttag acgcttctgg ttggacaatg
     6061 gtacgtttgt gtattaagta aggcttggct ggggatagca acattgggca gagtatagaa
     6121 gaccacaaaa aaaaggtata taagggcaga gaagtctttg taatgtgtgt aacttctctt
     6181 ccatgtgtaa tcagtatttc tacttacttc ttaaatatac agaagtaaga cagataacca
     6241 acagcctttc ccagatatac atatatatct ttatttcagc ttaaacaata attatatttg
     6301 tttaactcaa aaataaaaaa aaaaaaccaa actcacgcaa ctaattattc cataataaaa
     6361 taacaacatg cgtatcattg ttacaggtgc atctggactc cttggctccc agatatatgc
     6421 tgcattcaag aatacggaac atacggttct gggccttgcg cactcacgcg cgacaggaga
     6481 gctgcagaag ctagatctct tggatagcaa ggaggtcgag aaggtcttcg cagatttcaa
     6541 cccggaatgg gtgatacact gtgcggcgga gcgcaggcca gatgtagcgg agaagaaccc
     6601 cgacgggacg cggaaactta acgtcgaaat cccccttcac cttgcacaat tgtccaagtc
     6661 cctcaagtat acgctaatct acatctctac cgactatgtg ttcgatggaa cctcaccacc
     6721 ctacacccct ggtgcactca cgaaccctgt caacctctac ggccagacga agcgcgacgg
     6781 tgagctcgct gtcctcggcg tatctgacgc gcgtgcggtc gtcctgcgtg tgcctgtgct
     6841 ctacgggcct acgcccaaca atactgactc ggcggtgaac atccttctgg acatcgtcgc
     6901 ggaccagtcg ggcaagacat acaagatgga ccactacgca acgcgcttcc cgacgggcgt
     6961 cgtcgacatc gcggatttcc tcgtgaggct ggctacactc ccgccgtccc gacagatccc
     7021 accgatcctg cactactctg cgagtgagcc gttcaccaag tacgagatgt gcctcatctt
     7081 cgcgcgtatc cttggtgtgc cgcattcgca catcattccc gacgcagagg ctcccaaggg
     7141 tgatgcagca acgtcgcgcc ctcgcgattg tcagctgtat acccgcgaaa cggaagacct
     7201 gatggaggga tacggcggtc tcggctggac gccattcgag gagtggtggg tcgactatct
     7261 aaacaagagg cagtgaagtg cttttaacta agaattatta gtcttttctg cttatttttt
     7321 catcatagtt tagaacactt tatattaacg aatagtttat gaatctattt aggtttaaaa
     7381 attgatacag ttttataagt tactttttca aagactcgtg ctgtctattg cataatgcac
     7441 tggaaggaga aaaaaaaggt gcacacgcgt ggctttttct tgaatttgca gtttgaaaaa
     7501 taactacatg gatgataaga aaacatggag tacagtcact ttgagaacct tcaatcagct
     7561 ggtaacgtct tcgttaattg gatactcaaa aaagatggat agcatgaatc acaagatgga
     7621 aggaaatgcg ggccacgacc acagtgatat gcatatggga gatggagatg atacctccat
     7681 tgggccgatg aagttagtcg acggatagaa gcggttgtcc cctttcccgg cgagccggca
     7741 gtcgggccga ggttcggata aattttgtat tgtgttttga ttctgtcatg agtattactt
     7801 atgttctctt taggtaaccc caggttaatc aatcacagtt tcataccggc tagtattcaa
     7861 attatgactt ttcttctgca gtgtcagcct tacgacgatt atctatgagc tttgaatata
     7921 gtttgccgtg attcgtatct ttaattggat aataaaatgc gaaggatcga tgacccttat
     7981 tattattttt ctacactggc taccgattta actcatcttc ttgaaagtat ataagtaaca
     8041 gtaaaatata ccgtacttct gctaatgtta tttgtccctt atttttcttt tcttgtctta
     8101 tgctatagta cctaagaata acgactattg ttttgaacta aacaaagtag taaaagcaca
     8161 taaaagaatt aagaaaatgc tcccctcgct gagaaactac cttcggttta ttgatctcga
     8221 gaacgaattc gcggatcatg ggtttttgcc aaagcctgga gccggcttca agcttgtgca
     8281 aggaatccga gagagctgga ctgacttcac cgctctcgga cccggttacg gtacatggaa
     8341 cgtgatacgc tccgagatgg acgacatgat gctgcggcat gcatccaagc aaggcgtcaa
     8401 ggtcttcgaa gaaacccgtg tcgagaccct cgacttcgca ggggaccgcg cgtcttcgcg
     8461 cccgctcgct gcgcactggt cgaacaagca cggcgcagcg gggcgcatca cgttcgactg
     8521 gctcgtcgat gcctcggggc ggagcgggat catgtgcaca aagtacctca agaaccgcac
     8581 gatgcgcgag agtctgcgca atgtcgcggt gtggggctac tggaagggcg tgacgcgcta
     8641 cggcgagggc acgaagcgcg catgctcggg ctggttcgag gccctgacag atgaacaagg
     8701 gtggtcttgg accattccgt tgaatgacgg gacaacctcg attggcgtgg tgatgcatga
     8761 atctgcgtcg aaagcgaaga aggcccaggc caaggccgac ggccagaaac cgacccttac
     8821 ggagcactat ctcgaccagc tgcagtacct ccccggcgtg cgcgagctca tcggcgacgg
     8881 aggcgaattt atccccggga gcacgaagag cgccgcagac tacagctact cggcttccgc
     8941 gtattctggc gaccattttc gcgtcatcgg agacgcagcc aactttgtgg acccgttctt
     9001 ctcctccgga gttcacattg cgatgaccgg cgggttatct gcagcgataa caatctgcgc
     9061 atccatcagg ggtgacgccg atgagaatat tgcacaaagg tggcacgatg ccaaggttgg
     9121 aattgcgcat accagattcc tgttcgtcgt actcggcgcg taccagcaaa tgcacttgca
     9181 gtctgcccct gtcctgagcg atatcaatgc agaaaacttt gacgatgcat ttgagatgtt
     9241 ccgccccatc tacggcctcg cagacagcca gacaaggctc acagatgcaa aggtgcagca
     9301 catgatggat gtctgccaaa gcttcttcga cccatatgtc agcgaggagc atgtcctcgg
     9361 tgtacgtgca cgctatggac ctgcatctgc cacgcttgcc ggccccgtgc tgggttcgga
     9421 gcagatcgag cagatggtcg gtggtgacac cccagagcgt gagatggaca cgcgcgtgct
     9481 gcgcaagttt gatgcattga aagtgttcag tgacgatgtc gaggcgacat atatggagcg
     9541 aactgtcatt taactcctta agttacttta atgatttagt ttttattatt aataattcat
     9601 gctcatgaca tctcatatac acgtttataa aacttaaata gattgaaaat gtattaaaga
     9661 ttcctcaggg attcgatttt tttggaagtt tttgtttttt tttccttgag atgctgtagt
     9721 atttgggaac aattatacaa tcgaaagata tatgcttaca ttcgaccgtt ttagccgtga
     9781 tcattatcct atagtaacat aacctgaagc ataactgaca ctactatcat caatacttgt
     9841 cacatgagaa ctctgtgaat aattaggcca ctgaaatttg atgcctgaag gaccggcatc
     9901 acggattttc gataaagcac ttagtatcac actaattggc ttttcgctag tcttatctaa
     9961 aaattgcctt tatagtccgt ctctccagtc acggcctgtg taactgatta atcctgcctt
    10021 tctaatcacc attctactgt ttaattaagg gattttgtct tcatcaacgg cttccgccca
    10081 aaaaaaagta tgacgttttg cccgcaggcg tgaagctgcc catcttcacg ggcctgacct
    10141 cctctgccgg aacaccggcc atctccaact cataaattgg agaaataaga gaatttcaga
    10201 ttttcagagg atgaaaaaaa aaaggtagag agcataaaaa tggggttcac tttttggcaa
    10261 agttacagta tgcttattac atataaatag agtgccgata atggcttttt ttcatcttcg
    10321 aaatacgctt gctactgctc ttccagcgtt tttattactt ctttcttgtt tctccttagt
    10381 atataaaata tcaagctaca acaagcatac aatcaactgt caactgtcaa ttatattata
    10441 atacactatg tctgcgactg caggcatctc acaggtcgct ctgggaggct cagtgccccc
    10501 actcactgct catgctatct ctgtatctct gccgacatgg aatgacaacg ttgggtatga
    10561 gaagggcgag aagagggtcg tggatgcgat ggtcactggc tacccgcgtt tcttcatcca
    10621 tcgcagtatt caaaagctag ctcgtatctg cgagcagaag tttggcgcga acggtgaacg
    10681 atgcctgctg tgtccgactc gcaaagttgc agaggcgtgc cgctcctaca tgatcgatag
    10741 atctgcgaag gctggctcac aggtcactgt gcgcctcgtt caattcttca taagccccga
    10801 gggcgagaaa gattgcacca accgtagcat cgaactccat attgccctgt tcccgggcga
    10861 gtccttcccg tttgccaaag aattttggca gcacacaggt ttgtgcatct cgagccgtcg
    10921 tgctgagcgc tgcttgtcta tgttgccgga ggagagtccc gtctcgggat ctccgacgtc
    10981 cccatctctc gctcgcccgc ccaccaagcc tggaaataga cactactcta acaagagtct
    11041 cagctggact ccctcgacat cacctccatt gtcacagccg ccaggctcca cttccagatc
    11101 ccccgcagcg cctggagaga acctcagttt cgatcagacc acttacgtcg aggaacgcta
    11161 cggtcgcaac ttacccctcg agtccgctgc gttcacaaaa cgtgcactcc gccgccgcat
    11221 agcaggggtg ctcgtccgag acacccctag tgagtggtct actgcaggag cgcaagaggc
    11281 agaagttggt ccaagcacga gaggcgtgga aagtgtcaca gaagacgatg tcttcctcta
    11341 cccgaccggt atgtcggcga tctggagtgc tcatcagctg gcccttggta cacgtccaac
    11401 tgcaaagagt atctgctttg gattcccata tactgatact ctcaagattc tagagaagtg
    11461 gggacctggc tgtcacttct tgggctatgg cctcgattcg gacatagacg aactggagga
    11521 gatcctcagg cgagaatctg cagatacgac tcatccgcct gcattggcgc tcttcactga
    11581 gttcccttcc aacccgttgc tgcgctcggc ggatctgcca cgtctccgcg ccctggcaga
    11641 caaatatgac ttcctcatcg tcgttgacga gacgattggc aactttgtca acgtcgaagt
    11701 gctgccgtac gcagacgttg ttgtcagcag cctgagcaaa atcttcagcg gcgacgccaa
    11761 tgctatgggt ggcagcctgg tgctcaaccc acaaggccgc cactacgcag cgctcaaagc
    11821 tcacatggcg cagacgtacg aagacgtgta ctttgacgag gacgcgatct acatggaacg
    11881 caactcgcgc gatttccagc agcgcatcga ggtcgtcgac ggcaatacgc tagccgtctg
    11941 caccctcctg cgcgagcgct ccatcgtcgg gggctccacc gacgcgcaca ccgtcgtcaa
    12001 ggacgtgttc tacccacggt ggcagaccgc cgagcattac gagaagtgcc gccggccgga
    12061 cgggggctat ggcggactat tctcgatcac gttcacgacc accgcagcgt ctcgcgcgtt
    12121 cttcgacgca ctccgctgcc acaagggccc gagcctcggg acgaacttta cgctggcgtg
    12181 cccgtacacg attctggcgc acttcctgga gctggactgg gcggcgcagt atggcgtcga
    12241 ggagggcctt gtacgtgtca gcgtcggcat ggaggagaga caagaactgt tgaagatctt
    12301 cgaggatgcg ctgctcgcgg cagaggcgac agtggaaagc cagtgagcga atttcttatg
    12361 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
    12421 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
    12481 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
    12541 tctcaaatct tttagcgcca aggactccaa ctaattgtat cttgaatttg cctttacgat
    12601 ccgtttgtcc agtcacggca tgtatatctt attaatcctg cctttctaat cacgtattct
    12661 aatgttcaat taagggattt tatcttcatc aacggctccc acgcaaaaaa tgacgttttg
    12721 cacacagaca cgaaatacac cttccaccgg aacaacggcc atctccaact tataagttgg
    12781 ggaaataaga caatttcaga cttcagagaa tgaaaaaaaa aaaaggtaca tcacagatgg
    12841 ggttcaggtt tgctacaatt gcagggagcc tgtcacatat aaatagacct ccagtgatga
    12901 tatctttcag tcttcaaacg tctcttgtca cagttctggt cgttctatat cacatctctc
    12961 ttggttctac ttattgtcta taatatcaag ctacagcaag catacaatca actatctacc
    13021 ataccataat acacaatgaa ggctgtggta acaaacggaa acggggcatt tgggctgaag
    13081 tcagtcaagg ttccgacacc tgggcggggt gaggttctgg tgaaggtcat cgccgcatct
    13141 caaaatccaa ccgactggaa aacggctatg cttcatctca agcctggaaa catcttgggc
    13201 tgtgactttg cgggtgtcat agcagatatc ggcccagatg tcgacgcctc cttaatgata
    13261 acgcgcggca agcgcgtcgc aggagccgtt catggaggcg tcagcccaaa cggcgccttt
    13321 gccgaatacg tgacagtact ggcggacatg gtcatcgcga tcccagacaa gctttcattc
    13381 gaggacgctt cgcaactcgg catcgcttgc tacacgacat gccagtgtct ctaccaggaa
    13441 ctacatctac cgacgcctct ggaaccccta gaagtgccga tggacgttct catctggtcc
    13501 ggcacgtcga gcacaggtca atatgcagta cagtttgcca agctcgcagg gttgcgagtc
    13561 ctcagcacgg catctccgaa gaattttgaa cttgtaaaag atctaggtgc ggacgaagtc
    13621 ttcgactacg cagactcgaa gacagcacga aagatcttcg cggcgacgag tggcactctg
    13681 aaacacgctg tagattgtat cagcgagggc atgaccccga accaggtcag tatgtcgctg
    13741 agcaaggaag ggggaacgat tgccacgctt ttgccgtacg aaagccgtaa gaagggtgta
    13801 gagacacgat ttgttcttgc gtacaaattc tttggcaagg acatcgagtt ccctttcgtc
    13861 ttcccggcgg atcaagagca taggaagaat gctcctgtgt attgtagact catacaggaa
    13921 gtcatcacgt gctttgggct gaaggctacg ccggtgcgca tcttccctta cggcttggcg
    13981 agcgtctcag atggatttga gtatatgaga tccgggaagc tttctgcgga gaagatcaca
    14041 tacagaattt cggatacccc aggtctagat aagtgggatg tggccccgac tgggagtgtc
    14101 agaggttcct cgtgaacagg ccccttttcc tttgtcgata tcatgtaatt agttatgtca
    14161 cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg agttagacaa
    14221 cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta agaacgttat
    14281 ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt aacattatac
    14341 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc atttattgaa
    14401 aagtaaatat ctcgtaaccc ggatgctttg ggcggtcggg ttttgctact cgtcatccga
    14461 tgagaaaaac tgttcccttt tgccccaggt ttccattcat ccgagcgatc acttatctga
    14521 cttcgtcact ttttcatttc atccgaaaca atcaaaactg aagccaatca ccacaaaatt
    14581 aacactcaac gtcatctttc actacccttt acagaagaaa atatccatag tccggactag
    14641 catcccagta tgtgactcaa tattggtgca aaagagaaaa gcataagtca gtccaaagtc
    14701 cgcccttaac caggcacatc ggaattcaca aaacgtttct ttattatata aaggagctgc
    14761 ttcactggca aaattcttat tatttgtctt ggcttgctaa tttcatctta tccttttttt
    14821 cttttcacac ccaaatacct aacaattgag agaaaactct tagcataaca taacaaaaag
    14881 tcaacgaaaa atgcgggtct ggtacatcat gcattacgtc tactcattgc cacccagcgc
    14941 ggtccacatc agcaacaatc tatatacagt ggggaaatta gaccgtccgt cagtctcgct
    15001 gggacttgat tactttccaa cccgaacctt tccaaccctt cgccgtcgga gatggagcgg
    15061 aataaagcct ggctgcgggg cgctgcggga cataaaggag gacatggaga tatctctgat
    15121 caagccgaac ttggtacttc ggaagacacg gcacgacatc gcgttggtaa gcatggcgtt
    15181 gggtacggtg aacatcgtgc ggtatgtgac gggaacgccg gtcgcataca ccattgcgac
    15241 ggacaaagct atccgactca cacaaagtcg agttgtcaac cctgcaaaag cgtacgagag
    15301 aacgcaggaa gatactcccc cgtccactcg ggaattcatc aggagacgta ttgagattct
    15361 cgtgtacctg cgtcatgctg gattagcaga aactgaaatg aagcctctcg cggggctgct
    15421 gcacccatgc tatgttcaag aggctcagag gatcattcga tgtgatggct tcggcaagtc
    15481 cccatggatt acgcagcaca aacttggatg cgtttcgtct gaggacaaag ttgaacgagg
    15541 aaacccactg agaaccccgc tgtttacctc tgcgactgat cgactgcgaa agtgtacatc
    15601 cacgacatgt gaaatgcaca agggcccaga ctcttttaag acgcttcggg tcggctcacg
    15661 gtcccagtgg gcaagcgcaa tacatcgtca ggccgacgtt gtgcgagtat gggtggacag
    15721 catcgattct gcactcgctg cgacggagaa cctgccagtg gaggcgcagg aactgaatga
    15781 tgttcttgca ctcaatgagg acgagagtat cgggtgccag gtggagactc ggagaggatg
    15841 aggagattga taagactttt ctagttgcat atcttttata tttaaatctt atctattagt
    15901 taattttttg taatttatcc ttatatatag tctggttatt ctaaaatatc atttcagtat
    15961 ctaaaaattc ccctcttttt tcagttatat cttaacaggc gacagtccaa atgttgattt
    16021 atcccagtcc gattcatcag ggttgtgaag cattttgtca atggtcgaaa tcacatcagt
    16081 aatagtgcct cttacttgcc tcatagaatt tctttctctt aacgtcaccg tttggtcttt
    16141 tatagtttcg aaatctatgg tgataccaaa tggtgttccc aattcatcgt tacgggcgta
    16201 ttttttacca attgaagtat tggaatcgtc aattttaaag tatatctctc ttttacgtaa
    16261 agcctgcgag atcctcttaa gtatagcggg gaagccatcg ttattcgata ttgtcgtaac
    16321 aaatactttg atcggcgcta tgcggccgcc accgcggtgg agctccagct tttgttccct
    16381 ttagtgaggg ttaattgcgc gcttggcgag actcaaacaa gatatatttt gcccgcctta
    16441 cagacgaaac tagttggagg ttatggagca tactatcacg tgggcggcca ctggtgagtt
    16501 actacacccc aggggcaacg ttgatgctcc taaaaaactc tggctggacg caagccgtaa
    16561 cacccgtgtc acttcataat cgtttgcaat tcagggcttg atctacacat taccctgtta
    16621 tccctattaa ttaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    16681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    16741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    16801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    16861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    16921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    16981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    17041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    17101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    17161 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    17221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    17281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    17341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    17401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    17461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    17521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    17581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    17641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    17701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    17761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    17821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    17881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    17941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    18001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    18061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    18121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    18181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    18241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    18301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    18361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    18421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    18481 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
    18541 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
    18601 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
    18661 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
    18721 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
    18781 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
    18841 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
    18901 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
    18961 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
    19021 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
    19081 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
    19141 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
    19201 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
    19261 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
    19321 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
    19381 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
    19441 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
    19501 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
    19561 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
    19621 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
    19681 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
    19741 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
    19801 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
    19861 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    19921 agcgcgtttc ggtgatgacg gtgaaaacct cggcgcgcca ttaccctgtt atccctaata
    19981 tacaacgcaa agaagctgga aaattggcga gggaatcctg tttctgtcta tccaagaatg
    20041 ggcatgaggt ggcaaccgtc gtgctagcgt acagggtgca ctttgtaacc atttgggaca
    20101 ccggacactc gctgttttcg aaattaccct ttaagcgcgg gtattgaacc aggcttatgc
    20161 ccaagatcgt agcaagcatt taaatgacgg tatcgataag cttgatatcg aattcctgca
    20221 gcccggggga tccactagtt ctagattaat taatatctaa aaattgcctt atgatccgtc
    20281 tctccggtta cagcctgtgt aactgattaa tcctgccttt ctaatcacca ttctaatgtt
    20341 ttaattaagg gattttgtct tcattaacgg ctttcgctca taaaaatgtt atgacgtttt
    20401 gcccgcaggc gggaaaccat ccacttcacg agactgatct cctctgccgg aacaccgggc
    20461 atctccaact tataagttgg agaaataaga gaatttcaga ttgagagaat gaaaaaaaaa
    20521 aaaaaaaaaa
//
