LOCUS       sequence-22408-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Jen Y, Manova K, Benezra R
  TITLE     Expression patterns of Id1, Id2, and Id3 are highly related but 
            distinct from that of Id4 during mouse embryogenesis.
  JOURNAL   Dev Dyn. 1996 Nov . 207(3):235-52.
  PUBMED    8922523
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            1996 Nov . 207(3):235-52."
COMMENT     Sequence Label: pBS/KS-mId4sp
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(330..346)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(331..347)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(375..393)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(414..430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(414..430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(427..449)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    438..454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(462..468,469..486,487..492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    507..528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(645..662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggccgctct agactagtgg attcggggga aatcttcagc aaatctagaa acgtcgtctc
       61 gtcttgtcat tccaagagag agagagagag agagagaagg ggaaaaataa aacttaaatt
      121 cacttttact ttttttgcac gttcacgagc attcaccgta cgtattctct ttcgtttctt
      181 cttttatgac cgctgtgaat tgtacgtttc tgtggttatt tttatcaccc ttttgaaggt
      241 gcagttaaac ttcgaagctt aagtgttgtc gaccagactg ctaagtagaa gagcaatcgt
      301 gaatccaacg aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt
      361 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
      421 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      481 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      541 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      601 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      721 ttatccacag aatca
//
