LOCUS       sequence-224081-       15733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15733)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU37.2-2.2
FEATURES             Location/Qualifiers
     source          1..15733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2101..2400
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5366..5586
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5587..6390
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(11361..11381)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11361..11379)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(11718..12306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11798..11817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12477..13268,13269..13337))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13100..13119
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13338..13442)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13469..14811
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(14432..14479)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14852..14870
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15212..15228)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15212..15228)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acaatggttg cccctcctcc agactgggtc gcgaagttga cgccctccgg gccgcagggc
       61 agtgagctcc ttgcaacgga gcgtgcgaag agtaacatca acgttgagaa gctgtccaac
      121 ttcatgttcg gcaaggaggc gctggagaac gaggccaaga tcctggctat cctccagtcc
      181 gagaaggtgt tcgacaagtc gaacaatgcc ttcctgggaa gagtggagaa gttcccggtt
      241 gctctcgcca gagcgaagaa gctgagacag ctgcaagtca agcacaactg gagcaccgag
      301 gactacagaa tcgccgatgg cctgatctcc gagccaggcc catacgggct tcacgcctct
      361 atgttcttga ctgctctcag ggaccaagga acttcggaac aacacaagct cttccttgag
      421 ccggccgaga actacaagta catcggctgc tacgcccaga ccgagttggg acacggctcc
      481 aacgttcgtg gtcttgaaac caccgcgacc tgggacccta ccgacaagac cttcgtcatt
      541 cactctcctc atcttaccgc ctccaagtgg tggattggct ctttgggccg caccgccaac
      601 tacgccgtcg ttatggcaca gcttattctc aacggcaagt cctacggacc acaccccttc
      661 gttgtcccag tcagggactt gaagactcac cagccccttg acggcgtcta cgtcggagac
      721 attggaccaa aattcggtta caacaccatg gataacggtt tcctcctctt caacaaggtc
      781 aaggtacccc acgtcaacat gttggcaaag tactccagcg tcgacccaaa cacaaacaag
      841 tatgtccgcc cagcagctgc gtcgttggtt tacggaacta tgacctgggt tcgctccacc
      901 attgtgcttc aatccggcca gaccctggca agaggtgtca ctatcgccac caggtattgt
      961 gctgttcgca ggcaattcca ggaccgcgat gacatctcca acgatgtcac cgagaaccaa
     1021 gttctcaact acagcatggt gcaattccgt cttctccctc tcctcgcttc caccttcgct
     1081 ctccacttca ctggcaaggc catgatggaa atgtacgcac aaaaccaaga gaggatgaag
     1141 aaggctgttg ccaacaccag gagaggagcc ggcccagagg agcttcagtc tggcagtgat
     1201 atgcttgctg atctccacgc cacttcttgt ggacttaagg cgctcggcag caccatcgct
     1261 gctgagggtc tggaggtgtg cagaagagct tgcggtggcc acggttactc ccagttctct
     1321 ggtatcggca gcttctacgc cgattacctc ccaaccacca cctgggaggg agacaactac
     1381 atgttgaccc agcaagtcgc acgctacctc ctcaagattg cccgctccgt cctcaagggc
     1441 aaggcacccg tcaacgacac cgccgagatc ttccgcacct acttggacgc acaggacaag
     1501 ggctgcgcct tcgacatcct ctccaacgac ggcgacatcg tcgcagcctt cgcatggcgc
     1561 acatcctacc tcaccttcga ggccctcaag caccgcgacg gcgagaagaa gtcgtggaac
     1621 tccctcctcg tcgacttctg gcgcctgtcc actgcccact cgcagtacct cgtcgtcaag
     1681 aacttctacg acgccctgca gtcctcgtcg ctcacctacg agctcgactc cgtctccatc
     1741 accaccatgc accagctctt ccgcctcttc gccctcagca ccctcgagaa ggaggccgcc
     1801 gagttctact cctcctccgc cctcacagtc cgccagatcc agctcgtccg cacaaagtcg
     1861 gtcatgcagc tcctcgccga catcaggccg cacgccctgc gcctggttga cgcttggcag
     1921 ttcccggatt ggcagctcga cagcagtctg ggccgcaagg atggtaaggt ctacgaggat
     1981 atgttccaca gggccagcca gctgaacccg ctgaacggtc tgaccgtgga cccataccca
     2041 gagagtgatg tgttgatcaa gagcgatgag acgaaccgtg ctttgcaggc gaagttgtag
     2101 gcggatctct tatgtcttta cgatttatag ttttcattat caagtatgcc tatattagta
     2161 tatagcatct ttagatgaca gtgttcgaag tttcacgaat aaaagataat attctacttt
     2221 ttgctcccac cgcgtttgct agcacgagtg aacaccatcc ctcgcctgtg agttgtaccc
     2281 attcctctaa actgtagaca tggtagcttc agcagtgttc gttatgtacg gcatcctcca
     2341 acaaacagtc ggttatagtt tgtcctgctc ctctgaatcg tctccctcga tatttctcat
     2401 tttccttcgc atgccagcat tgaaatgatc gaagttcaat gatgaaacgg taattcttct
     2461 gtcatttact catctcatct catcaagtta tataattcta tacggatgta atttttcact
     2521 tttcgtcttg acgtccaccc tataatttca attattgaac cctcacgatc cagttctcca
     2581 gtgacacagc ctttatctgg tcaaaccttt ctttctaatc acctatgctg atgcttaatt
     2641 aagggatttt tgtctccatc aacggcatgc gcccaaaaat gacgtttttt ttaacccata
     2701 gacacgaaac tacccatttt ccaccggcct gacctaccac cggaacaacg gccatctcca
     2761 acttgcaagt tggggaaatt aagagcatcg caggtttaat ggaagaaaaa aaaaaggtac
     2821 agcacagcgc aaatggagtt agttccctta tgtcacacac tcacacacag tcggtcagat
     2881 caagcatact gggtgcgtat aaatagagtg gccattgcca ccctgtttat ctcaaaatct
     2941 gtcttgttag tggtcttctc cctttttcag gttacaattc tcttgtttct acttagtata
     3001 taagtatatc aagctatatt aagcatacta tcaactgtca actctatcct caaaatacaa
     3061 tacaaaatgt ccgaacctca gagcaatccc tctatcgagt tgaagagcgt ggccccagac
     3121 actttctcat cggattcgga gagggggttt gagcacaatg cgcaattgaa aaacactacc
     3181 gtcaagcatt tcatttggca agatgtcacg gtcacagtgc aggaccgggc cacaaagcag
     3241 tccaaggcaa tcttagagaa tctgaatgga gttgtctcag ctggcgagat gtgtgctttg
     3301 atgggcccga gtggttctgg aaagacaact ctgttgaact tcctcgccag acgcgacaac
     3361 cctggagctg atgccaccgg ctttgcatct gtaaatggaa acgccatatc cctcaatgag
     3421 ttcaggaaaa tgagctccta cgtggaacaa gaggatgcct tgattgggtc tttgacggtc
     3481 agagagacta tgaactttgc tgcacgcttg tcaaactctg gcctcacaaa gaaggatcgt
     3541 atcatgagaa tcgatggctt gattgattcg tttggtcttc ggaaacagga acacactatc
     3601 attggcacgc caattcgtca ggggattagc ggtgggcaga aacgccgtgt cagtgtagca
     3661 agccagttga ttacggcgcc aaagatctta ttcctggatg aaccaacaag cggcctcgac
     3721 tctgcggcta gcttcgaagt tgtttctttc attaagacct tggccaagca aaacaatttg
     3781 atcgtaattg ctagcataca ccagccgtcc acgcagacgt tctcactatt cgataagctg
     3841 ctcctcctgt cagagggtaa accgcactat tttggagctg tggacgatgt ggttccatac
     3901 tatctggaga tcgactaccc ggtgccattg catatgaacc ccgccgaaca tgcattagaa
     3961 ttgaccaaca aggattttgg atcgggcctg gtcgaggcag aagaagattt atccaagctc
     4021 cagtcagcct ggtcttctaa tccacgtgcc aaagcgattg ccagccagat caactcctat
     4081 ctcatcactg ccagcgaggg ccaaacacat aattcgaaga ccggctctca tgcaaacttt
     4141 gccgcggtca cattgaccct cgttcatcgc tcttttatca agtcgtaccg cgatgtcgtg
     4201 gcatacggaa ttcgcctagc aatgtacagc tgtcttgcta tcatgagcgg gacggtttgg
     4261 ctacgcctaa gcccttccca gacgtccatt caacctttca tcaacgccat atttttcgga
     4321 gcttcgtttt tatcgttcat ggctgtcgca tacgtgccgt cattcctcga agacaggtta
     4381 acttttatca aagagcgctc aaacggcctc tatggtgccg ctgccttcac agttgccaat
     4441 ttcatcattg gactcccata tctgtttggc attgcactca tatttacgtg catctcgtac
     4501 tggctgaccg gcttccataa taccgcccct gctttcttca cattcgtcat gtgggtattc
     4561 cttgacatgg tagccgcaga gagcttggta gtcctaatgt cctcgatctt tcccaacttc
     4621 gtcgtcgccc tcgctctcac agctttcgtg aacggtctgt ggatgtgcgt tggcggcttc
     4681 ctgatttcca cgcctctctt gaacgtgttc tggaaatacg tgttccacta catcgattac
     4741 caagcctatg tcttccaagg attgatggtc aacgagtttg ccgaccgcac gtacaactgt
     4801 gaccaggact gtcactgcat gtacaccacg gaactggcct cacaatgcat gatctctgga
     4861 aagggagtct tggacacgtt tgggatgtcc accggcctga caggcacgtg ggttgggatc
     4921 ttaattggaa ttgtagcagt gtatagactt cttggatggg ctgtgttggc actcaagcgt
     4981 taaacaaatc gctcttaaat atatacctaa agaacattaa agctatatta taagcaaaga
     5041 tacgtaaatt ttgcttatat tattatacac atatcatatt tctatatttt taagatttgg
     5101 ttatataatg tacgtaatgc aaaggaaata aattttatac attattgaac agcgtccaag
     5161 taactacatt atgtgcacta atagtttagc gtcgtgaaga ctttattgtg tcgcgaaaag
     5221 taaaaatttt aaaaattaga gcaccttgaa cttgcgaaaa aggttctcat caactgttta
     5281 aaaggaggat atcaggtcct atttctgaca aacaatatac aaatttagtt tcaaagatga
     5341 atcagtgcgc gaaggacata actcattcaa ttcaattcat catttttttt ttattctttt
     5401 ttttgatttc ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
     5461 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
     5521 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
     5581 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
     5641 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
     5701 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
     5761 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
     5821 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
     5881 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
     5941 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
     6001 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
     6061 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
     6121 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
     6181 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
     6241 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt
     6301 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca
     6361 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata
     6421 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga
     6481 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg ataggaaaaa accgagcttc
     6541 ctttcatccg gcgcggctgt gttctacata tcactgaagc tccgggtatt ttaagttata
     6601 caagggaaag atgccggcta gactagcaag ttttaggctg cttaacatta tggataggcg
     6661 gataaagggc ccaaacagga ttgtaaagct tagacgcttc tggttggaca atggtacgtt
     6721 tgtgtattaa gtaaggcttg gctggggata gcaacattgg gcagagtata gaagaccaca
     6781 aaaaaaaggt atataagggc agagaagtct ttgtaatgtg tgtaacttct cttccatgtg
     6841 taatcagtat ttctacttac ttcttaaata tacagaagta agacagataa ccaacagcct
     6901 ttcccagata tacatatata tctttatttc agcttaaaca ataattatat ttgtttaact
     6961 caaaaataaa aaaaaaaaac caaactcacg caactaatta ttccataata aaataacaac
     7021 atgaccgaac catcgccccc ggagaaaagt tcgccacagt cgccttctac tgctgcgcat
     7081 gttactccaa atgcttcaag tgaaggcgcg attgtcgact tcgatggcgc tggtgatcca
     7141 gcgaacccgt tgaactggtc gttttcaaga aaagtggtta cgagtggtct ttacagtctg
     7201 acaagccttg gatctgtgtg ggcaagcaca gcatactccc ctgccaatag caaggtcgcc
     7261 gatgagtttc atgtgtcacc agaagtagct atcatcggga cgagcctttt actcttgggc
     7321 tggggatttg gaccgttact ctgggcgccg ctgtcagaag tttacggccg gaaatggcca
     7381 gttttgatcc cattcttcat ctctattgtg atgtcatttg ggacggcgac ggcaaaggat
     7441 atccaaacga ttctgattac tcggttcttc accggattct ttggggctgc tcctatcaca
     7501 tgcacaggag gagtctatgt tgacatctgg gatgcatccc aacggggaaa tgctattgtt
     7561 gggtacacgc tcgccgtgtg tggaggtccg gctcttggac ccgtcattgg aggcgctatt
     7621 atttacagcg gcacaaattg gagatggact gaatatacta caggcatatt gcaagcctcg
     7681 atacttattc caggagtaat attcatcgaa gaatcatacg taccaatgtt actcgtcaga
     7741 aaggcacgca agctgagaca gaacacggga aactgggcac tgcacgccga gtgggaagaa
     7801 accggtgttt ccatcaaggc actcgccata aagttcggcc tacgacctct tcagatgcta
     7861 ttgacgccaa tttgcctctc ggttaccatc tactcatcat ttatatacgg cacattttat
     7921 gccagcctgg catcgttccc catcatcttc caagaaacgc gaggctggaa cgagctcgtc
     7981 gggtcgcttc cgttcctcgg agtgcttata ggaatcatta tgggcgcagc actcagcgct
     8041 ctgaaccaga aatactacaa catcgcgtac gaagctaacg acttcaaacc ggttccggag
     8101 tctcgcctcc cgccaatgat gattgcttca gtcgttttat cgggcggatt gttcatcatg
     8161 ggatggactt cttcccccga cctcccgtgg atagcaactt taataggagt cactatgatg
     8221 gggtttggat actatacgat ttttaccagc gccttgaatt atctcgtcga tacattccag
     8281 cggtggggtg ccagtgctat agccgcaaat acgttcatgc gaagtgtgct cgcggctgca
     8341 ttcccgctta ttgtcccgcc aatgttccga cgtctcggaa atgaatgggc attaagtgtg
     8401 ctcgccatct tctcggtgtt taatatcccg attccgttcg tcttttggtt ttatgggcct
     8461 cggatcagag cgatgggcaa atacagctcg aatatggggt agagtgcttt taactaagaa
     8521 ttattagtct tttctgctta ttttttcatc atagtttaga acactttatg ttaacgaata
     8581 gtttatgaat ctatttaggt ttaaaaattg atacagtttt ataagttact ttttcaaaga
     8641 ctcgtgctgt ctattgcata atgcactgga aggagaaaaa aaaggtgcac acgcgtggct
     8701 ttttcttgaa tttgcagttt gaaaaataac tacatggatg ataagaaaac atggagtaca
     8761 gtcactttga gaaccttcaa tcagctggta acgtcttcgt taattggata ctcaaaaaag
     8821 atggatagca tgaatcacaa gatggaagga aatgcgggcc acgaccacag tgatatgcat
     8881 atgggagatg gagatgatac ctccattggg ccgatgaagt tagtcgacgg atagaagcgg
     8941 ttgtcccctt tcccggcgag ccggcagtcg ggccgaggtt cggataaatt ttgtattgtg
     9001 ttttgattct gtcatgagta ttacttatgt tctctttagg taaccccagg ttaatcaatc
     9061 acagtttcat accggctagt attcaaatta tgacttttct tctgcagtgt cagccttacg
     9121 acgattatct atgagctttg aatatagttt gccgtgattc gtatctttaa ttggataata
     9181 aaatgcgaag gatcgatgac ccttattatt atttttctac actggctacc gatttaactc
     9241 atcttcttga aagtatataa gtaacagtaa aatataccgt acttctgcta atgttatttg
     9301 tcccttattt ttcttttctt gtcttatgct atagtaccta agaataacga ctattgtttt
     9361 gaactaaaca aagtagtaaa agcacataaa agaattaaga aaatgcggcc ccggctccca
     9421 ttcgacgatg ttgcttggga acaaagtgac aacgtttacg aagaatggcg aactaagctt
     9481 ctcaaaccag aaaatctaac ggaaatcgct ctttttgttg caaagtatca ccttgggacg
     9541 ccagtgaagg ttagaacgcc tcttacgggc gcctataatg tggcggtggt tgttgatttt
     9601 cgcggaggtg gttcgacatt gattcgcttt cccaaaccag gtaggctgat gtttcccgag
     9661 gagaaggtca ggaatgaagt cgccgtaatg cggtatattc aagctcatac gtcgatcccg
     9721 gtgccgttta tattgcactg gggcacgaag gaacagagtc cttgtggtct agggccgttt
     9781 attattatgg aatatgtcaa tcatgactgt gatatcagtg acgcgttgaa tacgccggga
     9841 ataccatctg aggaccgacc aattcttgat cctgctgtag atgaaggaag actccggaaa
     9901 ttatatgggc aaatggcaga tgttctgctt caactatcca tgttgtcatt ccctaaaata
     9961 ggctctctca ctcaagacga cgatgaagat actttgactg tggccgcaag accattgacg
    10021 gcaaatatga acgaggtcgt acagctcggg acattgccac gttcaaagct acctgataaa
    10081 agtgcagcct ttgaggcttc ttcgtcatac ctgactacac tagccaatat gcaccttcgc
    10141 cacttaacct accagcgaaa cgacgcgatt gtcagcgcga gcgactgctg ccaaaaatat
    10201 atcgcaagat acctcttccg aaagctttcc gttgaaggca agctgtcacg tcctgagcac
    10261 aatttcgggc cttttaaact ctggtgcgat gatctgcgcc cagctaatat gctaattaat
    10321 tccaacctcg agttggtttc cattattgac agggaatttg catatgctgc tcccgtggag
    10381 ttctcacatg cgcccccttg gtggctccta atagaacagc cagagcactg gtcggaagga
    10441 ctttacgcat gggctactgc ttatgaacct cggcttcaaa ctttcttggc cgttcttcgc
    10501 gagcgtgaag atgtcctcat agacgaggga agacttaaag accaggagag actctcgttg
    10561 cctatgcggg agagctggga cagtggtgat ttctgggtcg tatatgctgc tcgcaagggc
    10621 tttgcatttg atgcggtatt ctggaagtat ctggatgcac gcttctttgg ggctgtggat
    10681 ggagtggaag gggatgagtg ggaaaggaga gccgagttgc tggacaagga ggaacttatg
    10741 gaaattgaca gctttgtcga tcaaaaagtg gttgaaatga aaacccgagc cttagcatgg
    10801 gaacccgaag aacatcttgg ataaggagat tgataagact tttctagttg catatctttt
    10861 atatttaaat cttatctatt agttaatttt ttgtaattta tccttatata tagtctggtt
    10921 attctaaaat atcatttcag tatctaaaaa ttcccctctt ttttcagtta tatcttaaca
    10981 ggcgacagtc caaatgttga tttatcccag tccgattcat cagggttgtg aagcattttg
    11041 tcaatggtcg aaatcacatc agtaatagtg cctcttactt gcctcataga atttctttct
    11101 cttaacgtca ccgtttggtc ttttatagtt tcgaaatcta tggtgatacc aaatggtgtt
    11161 cccaattcat cgttacgggc gtatttttta ccaattgaag tattggaatc gtcaatttta
    11221 aagtatatct ctcttttacg taaagcctgc gagatcctct taagtatagc ggggaagcca
    11281 tcgttattcg atattgtcgt aacaaatact ttgatcggcg ctatgcggcc gccaccgcgg
    11341 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gagactcaaa
    11401 caagatatat tttgcccgcc ttacagacga aactagttgg aggttatgga gcatactatc
    11461 acgtgggcgg ccactggtga gttactacac cccaggggca acgttgatgc tcctaaaaaa
    11521 ctctggctgg acgcaagccg taacacccgt gtcacttcat aatcgtttgc aattcagggc
    11581 ttgatctaca cattaccctg ttatccctat taattaatac ggttatccac agaatcaggg
    11641 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    11701 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    11761 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11821 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11881 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11941 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    12001 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    12061 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    12121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    12181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    12241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    12301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    12361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    12421 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    12481 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    12541 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    12601 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    12661 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    12721 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    12781 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    12841 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    12901 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    12961 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13021 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    13081 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    13141 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    13201 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    13261 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    13321 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    13381 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    13441 cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca
    13501 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
    13561 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
    13621 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
    13681 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
    13741 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
    13801 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
    13861 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
    13921 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
    13981 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
    14041 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
    14101 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
    14161 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
    14221 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
    14281 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
    14341 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
    14401 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga
    14461 acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg
    14521 agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat
    14581 atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg
    14641 tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc
    14701 ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc
    14761 aattggatta gtctcatcct tcaatgctat catttccttt gatattggat catactaaga
    14821 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    14881 cgcgcgtttc ggtgatgacg gtgagcgcgt ttcggtgatg acggtgaaaa cctcggcgcg
    14941 ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc
    15001 ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt
    15061 gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg
    15121 cgggtattga accaggctta tgcccaagat cgtagcaagc atttaaatga cggtatcgat
    15181 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagatt aattaatatc
    15241 taaaaattgc cttatgatcc gtctctccgg ttacagcctg tgtaactgat taatcctgcc
    15301 tttctaatca ccattctaat gttttaatta agggattttg tcttcattaa cggctttcgc
    15361 tcataaaaat gttatgacgt tttgcccgca ggcgggaaac catccacttc acgagactga
    15421 tctcctctgc cggaacaccg ggcatctcca acttataagt tggagaaata agagaatttc
    15481 agattgagag aatgaaaaaa aaaaaaaaaa aaaaggcaga ggagagcata gaaatggggt
    15541 tcactttttg gtaaagctat agcatgccta tcacatataa atagagtgcc agtagcgact
    15601 tttttcacac tcgaaatact cttactactg ctctcttgtt gtttttatca cttcttgttt
    15661 cttcttggta aatagaatat caagctacaa aaagcataca atcaactatc aactattaac
    15721 tatatcgtaa tac
//
