LOCUS       sequence-224083-       13135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU35.2-2.2
FEATURES             Location/Qualifiers
     source          1..13135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1288..1587
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3593..3813
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3814..4617
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9045..9065)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9045..9063)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9402..9990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9482..9501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10161..10952,10953..11021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10784..10803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11022..11126)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11153..12495
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12116..12163)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12536..12554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12896..12912)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12896..12912)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
       61 aaaggcagag gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat
      121 cacatataaa tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc
      181 tctcttgttg tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa
      241 aagcatacaa tcaactatca actattaact atatcgtaat acacaatgga gaacgacaag
      301 ggcggatata ggcagatcaa taagtcgttg aacatctgtg cctttgatga gtatcttaaa
      361 ggtcagaccg cacgactgcc gcatatcgag agtattgaac agcttactcc aagagtgctt
      421 cgcgttcttg gccagaaccc tggaaagttt acgcatcaag ggacaaacac atacatcgtc
      481 ggcactggcc aacgacgcct catcattgat accggtgggg gcgagccagc atgggccaaa
      541 ctgattgact caacatttat atcaatgaac atcagcctct catatgttct gctgacccat
      601 tggcacggtg accataccgg cggagttcca gctcttctgc gcctataccc acatctcacg
      661 aattccatct acaagaacga accggaccaa agccaacaga acatcaccca cggacagata
      721 ttttctgttg acggcgcaac aatccgtgct attcatgtgc ctggacattc agaagatcac
      781 atgtgtttta tcttagaaga ggagcaggca atgtttaccg gtgacaatat ccttggccac
      841 ggtaccagtg ctgtagaaga cctcggcacg ttcatggcca gcctacagaa gatggctgcc
      901 caggattgcc atatcggtta ttctgctcat ggcgccacag tcaccgactt gccagccaaa
      961 attaccgggg aattagataa caagttgcgg cgcgagaagc aagttatgaa tgcgcttggc
     1021 agagtccgta gccgcggcga gaagagcgtc accaccaaag acttggtaac agagatctac
     1081 ggcgagtcgc ttgacgaaga tacgaggata ctcgcattgg agccatttat cgacgaagtg
     1141 ctacggaaac tagcgaatga ctgtaaggtt gcattcgaaa tgaggagagg caagaagaag
     1201 tggttctctg tggaggaaat ggaggagaca accacgcagc ggtttatggc tctgaaaaat
     1261 ccactggccg taactattca agtttaagcg gatctcttat gtctttacga tttatagttt
     1321 tcattatcaa gtatgcctat attagtatat agcatcttta gatgacagtg ttcgaagttt
     1381 cacgaataaa agataatatt ctactttttg ctcccaccgc gtttgctagc acgagtgaac
     1441 accatccctc gcctgtgagt tgtacccatt cctctaaact gtagacatgg tagcttcagc
     1501 agtgttcgtt atgtacggca tcctccaaca aacagtcggt tatagtttgt cctgctcctc
     1561 tgaatcgtct ccctcgatat ttctcatttt ccttcgcatg ccagcattga aatgatcgaa
     1621 gttcaatgat gaaacggtaa ttcttctgtc atttactcat ctcatctcat caagttatat
     1681 aattctatac ggatgtaatt tttcactttt cgtcttgacg tccaccctat aatttcaatt
     1741 attgaaccct cacgatccag ttctccagtg acacagcctt tatctggtca aacctttctt
     1801 tctaatcacc tatgctgatg cttaattaag ggatttttgt ctccatcaac ggcatgcgcc
     1861 caaaaatgac gtttttttta acccatagac acgaaactac ccattttcca ccggcctgac
     1921 ctaccaccgg aacaacggcc atctccaact tgcaagttgg ggaaattaag agcatcgcag
     1981 gtttaatgga agaaaaaaaa aaggtacagc acagcgcaaa tggagttagt tcccttatgt
     2041 cacacactca cacacagtcg gtcagatcaa gcatactggg tgcgtataaa tagagtggcc
     2101 attgccaccc tgtttatctc aaaatctgtc ttgttagtgg tcttctccct ttttcaggtt
     2161 acaattctct tgtttctact tagtatataa gtatatcaag ctatattaag catactatca
     2221 actgtcaact ctatcctcaa aatacaatac aaaatgactt tccatcccga cactctaccc
     2281 gtcctcaagg gcaaagtatt catagtcacc ggtgggaact ccggcatagg ctactatacc
     2341 gtatcccacc tagcagaaca tggcgcccac gtctacatgt gcgcccgatc cccaaagaag
     2401 ggcacagaag ccatcagaaa catcaagaaa atgcacccct cagccaatat cgacctcctg
     2461 caaatggacc tcatggacct caccagcgtc gtcgcagccg ccaagttatt catcaccctc
     2521 gaaacaaccc tgcatggcct ggtcaacaat gcaggcatca tggctacgcc ttttgaaatg
     2581 acgaaggatg gttacgaggc ccagtggcag accaactacc tctcccactg ggttttgacg
     2641 gagcatcttc tccctctcat gttgcagact tccaagacgc tttctcctgg cagcgtccgc
     2701 atcgtcaatc taacttcatc gggtcacttg agcgcaccca agggcggtat caatttcgat
     2761 gatctatcac tcaaggacag cggcccatgg tcacgatacg gacagagcaa gctggccaat
     2821 attctgcaca ccaagactct gcaaaattcg tacggccccg gttctcccag tgcgcagaac
     2881 ggcgggggcg agatctgggt ttcgtctgtg catcccggcg tggttgatac gaaccttgcc
     2941 acatcggtgg aggagtccgg gacaggcatt tcgagtcttc tctcaatcat gcgcatggtc
     3001 ggattgatat ggcctgcgga taaggggtcg tggaatagtg tgttttgtgc ggctagtcag
     3061 gatatgacgg cggagcagag cggggcgtat atggagatct ttaagcgttt tggagagctg
     3121 aggtgccaga gtggtgcggc gaaggatgag cagcttgcgg agaggttaga ggagtggact
     3181 agggtggcga tggctgtggt ttctaagtga acaaatcgct cttaaatata tacctaaaga
     3241 acattaaagc tatattataa gcaaagatac gtaaattttg cttatattat tatacacata
     3301 tcatatttct atatttttaa gatttggtta tataatgtac gtaatgcaaa ggaaataaat
     3361 tttatacatt attgaacagc gtccaagtaa ctacattatg tgcactaata gtttagcgtc
     3421 gtgaagactt tattgtgtcg cgaaaagtaa aaattttaaa aattagagca ccttgaactt
     3481 gcgaaaaagg ttctcatcaa ctgtttaaaa ggaggatatc aggtcctatt tctgacaaac
     3541 aatatacaaa tttagtttca aagatgaatc agtgcgcgaa ggacataact cattcaattc
     3601 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     3661 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     3721 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     3781 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     3841 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     3901 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     3961 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     4021 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     4081 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     4141 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     4201 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     4261 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     4321 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     4381 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     4441 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     4501 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     4561 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     4621 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     4681 tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4741 catcaggata ggaaaaaacc gagcttcctt tcatccggcg cggctgtgtt ctacatatca
     4801 ctgaagctcc gggtatttta agttatacaa gggaaagatg ccggctagac tagcaagttt
     4861 taggctgctt aacattatgg ataggcggat aaagggccca aacaggattg taaagcttag
     4921 acgcttctgg ttggacaatg gtacgtttgt gtattaagta aggcttggct ggggatagca
     4981 acattgggca gagtatagaa gaccacaaaa aaaaggtata taagggcaga gaagtctttg
     5041 taatgtgtgt aacttctctt ccatgtgtaa tcagtatttc tacttacttc ttaaatatac
     5101 agaagtaaga cagataacca acagcctttc ccagatatac atatatatct ttatttcagc
     5161 ttaaacaata attatatttg tttaactcaa aaataaaaaa aaaaaaccaa actcacgcaa
     5221 ctaattattc cataataaaa taacaacatg gatagattga cggaactcaa tggtcatagc
     5281 gatcagctcg cccaagctat caggagcctc gtcgatagcc gtcgaggagc cgattcttct
     5341 gaccagctgg ggccaagtgc tgacactgat gtagacagag atcaatcttt attcacaaga
     5401 gccaagtcaa atattctggc cagtattgca gcaatcaagt ccatagttgg tgaaccagca
     5461 gacttcctcc aggatttttc tcgccagatc gaaatccttg cttgtcttcg gtggctagcg
     5521 gaattccaag tttttgcgtg catcccgttt gacgaaagct tgcctatcaa ggatttggcg
     5581 gacctagtag gagttcccga gagccagttg gttcgtgtca tccgtctgac tgccacatgc
     5641 ggatttctgc gggaaccgat actaggcttt gtgtcccaca gccccctctc ggcgcaattc
     5701 atatccaacc agtcattagt tgacgctatg gtgtttattg ccgagttggc tactcccaca
     5761 gcattgcaga tgccgcaagc aacgcagcga tttggagcct cgtcgagtgc caccgagagc
     5821 gcatacaact tagctctcaa tacggtgcgg ccgtttcatg tggctatcca agaacgctct
     5881 aagctgcgtc gtcaatggtc agcttacctt tgccatgcag cagggttgca ccaggaggaa
     5941 gaaatcatcg aagtgttatc gcggctcaac tggtctaacc ttggtaatgc gtgcatagtc
     6001 gaggtagggg caaagtcgac ctcgatggca agctctcttt ccaaaatgtt tccaagcttg
     6061 cggttggtcg tgcaaatcga caatacacaa tcttctttgc tagatcgaga ctacatgtgg
     6121 caaaatgcag ttctggcaag ctcaatccgg gacagctcta gctctggatc tagcccttct
     6181 cccacaaacc cttttatcac ggttacctat catgccatgg gcatgccgca gtctgtgaca
     6241 gatgctgccg tttatattat tcacgtgcct gccacatcct caggattgac gattaaagca
     6301 gaactggatg attacttagg tgtgcttcgt accagcgggg gcatcatgct tatcttgacg
     6361 acacgactgc ttcccgagcc aggcactctt cccaacccag agatcgaggc cgtggcgcgc
     6421 gcacgggatc tgagcatgct ccaactggcc aacgagagcg agatggagat gtccgagctt
     6481 ctgagtataa taaagacggt gggggacaac gctgggaaac tcgttgtcac taatcacctt
     6541 cgctcaaaca atggcgtcat cctagcttta actatcaagt accaagcata ttaaagtgct
     6601 tttaactaag aattattagt cttttctgct tattttttca tcatagttta gaacacttta
     6661 tattaacgaa tagtttatga atctatttag gtttaaaaat tgatacagtt ttataagtta
     6721 ctttttcaaa gactcgtgct gtctattgca taatgcactg gaaggagaaa aaaaaggtgc
     6781 acacgcgtgg ctttttcttg aatttgcagt ttgaaaaata actacatgga tgataagaaa
     6841 acatggagta cagtcacttt gagaaccttc aatcagctgg taacgtcttc gttaattgga
     6901 tactcaaaaa agatggatag catgaatcac aagatggaag gaaatgcggg ccacgaccac
     6961 agtgatatgc atatgggaga tggagatgat acctccattg ggccgatgaa gttagtcgac
     7021 ggatagaagc ggttgtcccc tttcccggcg agccggcagt cgggccgagg ttcggataaa
     7081 ttttgtattg tgttttgatt ctgtcatgag tattacttat gttctcttta ggtaacccca
     7141 ggttaatcaa tcacagtttc ataccggcta gtattcaaat tatgactttt cttctgcagt
     7201 gtcagcctta cgacgattat ctatgagctt tgaatatagt ttgccgtgat tcgtatcttt
     7261 aattggataa taaaatgcga aggatcgatg acccttatta ttatttttct acactggcta
     7321 ccgatttaac tcatcttctt gaaagtatat aagtaacagt aaaatatacc gtacttctgc
     7381 taatgttatt tgtcccttat ttttcttttc ttgtcttatg ctatagtacc taagaataac
     7441 gactattgtt ttgaactaaa caaagtagta aaagcacata aaagaattaa gaaaatggac
     7501 gccttcagag actctactcc tcaggaatct gcctattccg cccctcaggt tgccgcctgg
     7561 ctcggtcgca tctccctccc ctcatcccta acccaatacg tggattctcc gtcagccttt
     7621 cctaagacag ccacatccct ccagtcgctg ttccgatgcc agatcacaac ctttccttac
     7681 gagaatctct cagtgcacta ctcccagagc caccaggtca acatccagcc agatgtgctg
     7741 tttacgaaaa tgatgggccc ggatcataac ggcaggggcg gctactgcat ggaacttagc
     7801 atcttcttcc accacatgct gcgcgggctt gggttccacg tctacatgac gggcgtgtgc
     7861 aaccggacgc ggactgacgg caagccgcag ggcgaatatc aggggtggac gcacattaac
     7921 aacatcgtcc acctcccttg cggctccaag tttagcgccg acgtcgcgtt tggtggtgac
     7981 gggccgacgt ctcctcttcc tatggatggc atggcatctg cgcttcgcaa tctaggaacc
     8041 caagaggtac gcctcataca ggaaaacatt cctaagcagc gccttcggga gccgaagttg
     8101 tgggtgtacc agtatcgcaa cagttctgat aaggagtgga actcattcta cagcttttct
     8161 gagatcgagt tctttcagga ggattttgag gttcagaatt ggtggactag cgctaagaca
     8221 ctccatcggt ggactgtgtt agtggttcga tttctaaggg agggtgagcc cattaaattc
     8281 tctagagatg gtgcctggag gaaaggagat gaccaagaga taagcattgt cggcaaggta
     8341 atgctagtaa ataacctggt taaggttaat gtagggggga agacaagggt tgtacatgag
     8401 tttaattccg aggatggacg gttgctggca ctccaggagt attttgggat tacattaacg
     8461 caggaggagg cccagttcat acaggggtgg gacatggcgc taaaataggg agattgataa
     8521 gacttttcta gttgcatatc ttttatattt aaatcttatc tattagttaa ttttttgtaa
     8581 tttatcctta tatatagtct ggttattcta aaatatcatt tcagtatcta aaaattcccc
     8641 tcttttttca gttatatctt aacaggcgac agtccaaatg ttgatttatc ccagtccgat
     8701 tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca catcagtaat agtgcctctt
     8761 acttgcctca tagaatttct ttctcttaac gtcaccgttt ggtcttttat agtttcgaaa
     8821 tctatggtga taccaaatgg tgttcccaat tcatcgttac gggcgtattt tttaccaatt
     8881 gaagtattgg aatcgtcaat tttaaagtat atctctcttt tacgtaaagc ctgcgagatc
     8941 ctcttaagta tagcggggaa gccatcgtta ttcgatattg tcgtaacaaa tactttgatc
     9001 ggcgctatgc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     9061 attgcgcgct tggcgagact caaacaagat atattttgcc cgccttacag acgaaactag
     9121 ttggaggtta tggagcatac tatcacgtgg gcggccactg gtgagttact acaccccagg
     9181 ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa gccgtaacac ccgtgtcact
     9241 tcataatcgt ttgcaattca gggcttgatc tacacattac cctgttatcc ctattaatta
     9301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc
    11161 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
    11221 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
    11281 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
    11341 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
    11401 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
    11461 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
    11521 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
    11581 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
    11641 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
    11701 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
    11761 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
    11821 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
    11881 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
    11941 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
    12001 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
    12061 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
    12121 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
    12181 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
    12241 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
    12301 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
    12361 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
    12421 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
    12481 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag
    12541 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgagc gcgtttcggt
    12601 gatgacggtg aaaacctcgg cgcgccatta ccctgttatc cctaatatac aacgcaaaga
    12661 agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc aagaatgggc atgaggtggc
    12721 aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt tgggacaccg gacactcgct
    12781 gttttcgaaa ttacccttta agcgcgggta ttgaaccagg cttatgccca agatcgtagc
    12841 aagcatttaa atgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
    12901 actagttcta gattaattaa tatctaaaaa ttgccttatg atccgtctct ccggttacag
    12961 cctgtgtaac tgattaatcc tgcctttcta atcaccattc taatgtttta attaagggat
    13021 tttgtcttca ttaacggctt tcgctcataa aaatgttatg acgttttgcc cgcaggcggg
    13081 aaaccatcca cttcacgaga ctgatctcct ctgccggaac accgggcatc tccaa
//
